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- W2055961605 abstract "Bacteriophage 63D, previously isolated from sewage, is associated with α-2,8-linked polysialic acid degrading activity. We cloned a DNA fragment containing the sialidase gene from a 63D phage genomic library and the enzyme was functionally expressed in Escherichia coli. Determination of the nucleotide sequence of the fragment revealed that it contained one open reading frame (ORF) coding for a 108-kDa polypeptide consisting of 984 amino acid residues. The fragment had promoter sequences similar to the E. coli consensus promoters for σ70. The deduced amino acid sequence of the central region of the ORF showed homology to those of phages K1F (51.6% identity) and PK1E (51.7% identity) endosialidases. Two Asp-box motifs that are widely found in sialidases were conserved. Purification of the soluble enzyme from lysed culture broth of infected E. coli yielded a 90-kDa protein upon SDS polyacrylamide gel electrophoresis, suggesting that the primary translational product is processed to the mature 90-kDa protein. The molecular mass of the enzyme was determined as 360 kDa by gel filtration, indicating that the native enzyme was probably a tetramer of identical 90-kDa subunits." @default.
- W2055961605 created "2016-06-24" @default.
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- W2055961605 date "2000-01-01" @default.
- W2055961605 modified "2023-09-26" @default.
- W2055961605 title "Molecular cloning and characterization of a novel bacteriophage-associated sialidase" @default.
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- W2055961605 doi "https://doi.org/10.1016/s1389-1723(00)80035-3" @default.
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