FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056032683> ?p ?o ?g. }

• W2056032683 endingPage "19966" @default.
• W2056032683 startingPage "19957" @default.
• W2056032683 abstract "The nonsterile environment of the oral cavity facilitates substantial proteolytic processing, not only of resident salivary proteins but also of dietary proteins. To gain insight into whole saliva enzymatic processes, the in vivo generated peptides in this oral fluid were subjected to nano-flow liquid chromatography electrospray ionization tandem mass spectrometry. The 182 peptides identified were predominantly derived from acidic and basic proline-rich proteins, statherin, and histatins. The proteolytic cleavages in the basic proline-rich proteins occurred preferentially after a Gln residue with predominant specificity for the tripeptide Xaa-Pro-Gln, where Xaa in the P(3) position was mostly represented by Lys. Using the synthetic substrates Lys-Pro-Gln-pNA and Gly-Gly-Gln-pNA, the overall K(m) values were determined to be 97 +/- 7.7 and 611 +/- 28 microm, respectively, confirming glutamine endoprotease activity in whole saliva and the influence of the amino acids in positions P(2) and P(3) on protease recognition. The pH optimum of Lys-Pro-Gln-pNA hydrolysis was 7.0, and the activity was most effectively inhibited by antipain and 4-(2-aminoethyl) benzenesulfonyl fluoride, was metal ion-dependent, and not inhibited by cysteine protease inhibitors. A systematic evaluation of enzyme activities in various exocrine and nonexocrine contributors to whole saliva revealed that the glutamine endoprotease is derived from dental plaque and likely microbial in origin. The P(1) site being occupied by a Gln residue is a nonarchetype with respect to known proteases and indicates the presence of novel glutamine-specific endoprotease(s) in oral fluid." @default.
• W2056032683 created "2016-06-24" @default.
• W2056032683 creator A5007406317 @default.
• W2056032683 creator A5013657144 @default.
• W2056032683 creator A5055460311 @default.
• W2056032683 creator A5057672280 @default.
• W2056032683 date "2008-07-01" @default.
• W2056032683 modified "2023-09-28" @default.
• W2056032683 title "Identification of Lys-Pro-Gln as a Novel Cleavage Site Specificity of Saliva-associated Proteases" @default.
• W2056032683 cites W1480452288 @default.
• W2056032683 cites W1481910818 @default.
• W2056032683 cites W149228199 @default.
• W2056032683 cites W1492833857 @default.
• W2056032683 cites W1501170818 @default.
• W2056032683 cites W1547429501 @default.
• W2056032683 cites W1560391076 @default.
• W2056032683 cites W1768001471 @default.
• W2056032683 cites W1921974795 @default.
• W2056032683 cites W1973891497 @default.
• W2056032683 cites W1983405992 @default.
• W2056032683 cites W1996548593 @default.
• W2056032683 cites W1998359381 @default.
• W2056032683 cites W2000053133 @default.
• W2056032683 cites W2008683084 @default.
• W2056032683 cites W2012717554 @default.
• W2056032683 cites W2014323927 @default.
• W2056032683 cites W2036935356 @default.
• W2056032683 cites W2043255804 @default.
• W2056032683 cites W2043472552 @default.
• W2056032683 cites W2045514872 @default.
• W2056032683 cites W2045530758 @default.
• W2056032683 cites W2046741524 @default.
• W2056032683 cites W2048666997 @default.
• W2056032683 cites W2049277493 @default.
• W2056032683 cites W2064245793 @default.
• W2056032683 cites W2066653615 @default.
• W2056032683 cites W2067280609 @default.
• W2056032683 cites W2077114229 @default.
• W2056032683 cites W2090470556 @default.
• W2056032683 cites W2098561534 @default.
• W2056032683 cites W2098641091 @default.
• W2056032683 cites W2100250996 @default.
• W2056032683 cites W2111301002 @default.
• W2056032683 cites W2132068224 @default.
• W2056032683 cites W2138452485 @default.
• W2056032683 cites W2144627189 @default.
• W2056032683 cites W2156747855 @default.
• W2056032683 cites W2166383550 @default.
• W2056032683 cites W2169455272 @default.
• W2056032683 cites W2170710712 @default.
• W2056032683 cites W2282297600 @default.
• W2056032683 cites W4244066856 @default.
• W2056032683 doi "https://doi.org/10.1074/jbc.m708282200" @default.
• W2056032683 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2459269" @default.
• W2056032683 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18463091" @default.
• W2056032683 hasPublicationYear "2008" @default.
• W2056032683 type Work @default.
• W2056032683 sameAs 2056032683 @default.
• W2056032683 citedByCount "60" @default.
• W2056032683 countsByYear W20560326832012 @default.
• W2056032683 countsByYear W20560326832013 @default.
• W2056032683 countsByYear W20560326832014 @default.
• W2056032683 countsByYear W20560326832015 @default.
• W2056032683 countsByYear W20560326832016 @default.
• W2056032683 countsByYear W20560326832017 @default.
• W2056032683 countsByYear W20560326832018 @default.
• W2056032683 countsByYear W20560326832020 @default.
• W2056032683 countsByYear W20560326832021 @default.
• W2056032683 countsByYear W20560326832023 @default.
• W2056032683 crossrefType "journal-article" @default.
• W2056032683 hasAuthorship W2056032683A5007406317 @default.
• W2056032683 hasAuthorship W2056032683A5013657144 @default.
• W2056032683 hasAuthorship W2056032683A5055460311 @default.
• W2056032683 hasAuthorship W2056032683A5057672280 @default.
• W2056032683 hasBestOaLocation W20560326831 @default.
• W2056032683 hasConcept C167392928 @default.
• W2056032683 hasConcept C181199279 @default.
• W2056032683 hasConcept C182220744 @default.
• W2056032683 hasConcept C185592680 @default.
• W2056032683 hasConcept C2776714187 @default.
• W2056032683 hasConcept C2779349466 @default.
• W2056032683 hasConcept C504460877 @default.
• W2056032683 hasConcept C515207424 @default.
• W2056032683 hasConcept C55493867 @default.
• W2056032683 hasConceptScore W2056032683C167392928 @default.
• W2056032683 hasConceptScore W2056032683C181199279 @default.
• W2056032683 hasConceptScore W2056032683C182220744 @default.
• W2056032683 hasConceptScore W2056032683C185592680 @default.
• W2056032683 hasConceptScore W2056032683C2776714187 @default.
• W2056032683 hasConceptScore W2056032683C2779349466 @default.
• W2056032683 hasConceptScore W2056032683C504460877 @default.
• W2056032683 hasConceptScore W2056032683C515207424 @default.
• W2056032683 hasConceptScore W2056032683C55493867 @default.
• W2056032683 hasIssue "29" @default.
• W2056032683 hasLocation W20560326831 @default.
• W2056032683 hasLocation W20560326832 @default.
• W2056032683 hasLocation W20560326833 @default.
• W2056032683 hasLocation W20560326834 @default.

• next