FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056098585> ?p ?o ?g. }

• W2056098585 endingPage "88" @default.
• W2056098585 startingPage "83" @default.
• W2056098585 abstract "Background During induction of apoptosis, the pro-apoptotic member of the Bcl-2 protein family (Bax) undergoes translocation to the mitochondria. The translocation, which leads to accumulation of Bax in the mitochondrial intermembrane space, appears to be the critical event determining release of cytochrome c to cytosol: the latter event triggers the irreversible steps of apoptosis, namely, the activation of caspases and the initiation of the degradation of many proteins. The aim of this study was to utilize the morphometric capabilities of the laser scanning cytometer (LSC) and adapt this instrument to detect and measure in situ the process of translocation of Bax to mitochondria. Methods Human breast carcinoma MCF-7 cells growing on microscope slides were treated with the DNA topoisomerase I inhibitor, camptothecin (CPT). CPT is known to induce apoptosis preferentially of S-phase cells. The cells were fixed and permeabilized on the slides, their DNA was stained with propidium iodide (PI), Bax was detected immunocytochemically with the fluoresceinated antibody, and red and green fluorescence emission was measured by the LSC. Results Prior to induction of apoptosis, Bax was uniformly and diffusely dispersed in the cell nucleus and cytoplasm. Its translocation and accumulation in mitochondria in cells undergoing apoptosis were detected and measured by the LSC as the increase in intensity of maximal pixel of Bax immunofluorescence. Bivariate analysis of DNA content versus maximal pixel of Bax fluorescence revealed that the CPT-induced Bax translocation into mitochondria was preferential to S-phase cells. Total cellular Bax immunofluorescence measured by flow cytometry, however, was increased in all phases of the cycle without a preference to S-phase cells. Conclusion Changes in abundance and localization of particular proteins that undergo translocation within the cell, leading to their altered local density, may be conveniently detected by the LSC by taking advantage of its morphometric capabilities. Measurement of total cellular Bax immunofluorescence by flow cytometry combined with analysis of its translocation by LSC revealed that apoptosis of S-phase cells induced by CPT was unrelated to overall Bax abundance per cell but correlated with its accumulation in mitochondria. Cytometry 41:83–88, 2000 © 2000 Wiley-Liss, Inc." @default.
• W2056098585 created "2016-06-24" @default.
• W2056098585 creator A5015640756 @default.
• W2056098585 creator A5059874954 @default.
• W2056098585 creator A5085243647 @default.
• W2056098585 creator A5086304089 @default.
• W2056098585 date "2000-01-01" @default.
• W2056098585 modified "2023-10-11" @default.
• W2056098585 title "Translocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry" @default.
• W2056098585 cites W1537149667 @default.
• W2056098585 cites W1966112176 @default.
• W2056098585 cites W1967912613 @default.
• W2056098585 cites W1975667451 @default.
• W2056098585 cites W1979995768 @default.
• W2056098585 cites W1991253583 @default.
• W2056098585 cites W1994659268 @default.
• W2056098585 cites W2002092281 @default.
• W2056098585 cites W2008901624 @default.
• W2056098585 cites W2009530630 @default.
• W2056098585 cites W2036822121 @default.
• W2056098585 cites W2036937264 @default.
• W2056098585 cites W2040955353 @default.
• W2056098585 cites W2052581310 @default.
• W2056098585 cites W2053509579 @default.
• W2056098585 cites W2073433611 @default.
• W2056098585 cites W2086118963 @default.
• W2056098585 cites W2108131089 @default.
• W2056098585 cites W2134872883 @default.
• W2056098585 cites W2149967205 @default.
• W2056098585 cites W2151501496 @default.
• W2056098585 cites W2158398965 @default.
• W2056098585 cites W2165436746 @default.
• W2056098585 cites W4240505428 @default.
• W2056098585 cites W4241419378 @default.
• W2056098585 doi "https://doi.org/10.1002/1097-0320(20001001)41:2<83::aid-cyto1>3.0.co;2-r" @default.
• W2056098585 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11002262" @default.
• W2056098585 hasPublicationYear "2000" @default.
• W2056098585 type Work @default.
• W2056098585 sameAs 2056098585 @default.
• W2056098585 citedByCount "61" @default.
• W2056098585 countsByYear W20560985852012 @default.
• W2056098585 countsByYear W20560985852013 @default.
• W2056098585 countsByYear W20560985852014 @default.
• W2056098585 countsByYear W20560985852015 @default.
• W2056098585 countsByYear W20560985852016 @default.
• W2056098585 countsByYear W20560985852017 @default.
• W2056098585 countsByYear W20560985852018 @default.
• W2056098585 countsByYear W20560985852021 @default.
• W2056098585 crossrefType "journal-article" @default.
• W2056098585 hasAuthorship W2056098585A5015640756 @default.
• W2056098585 hasAuthorship W2056098585A5059874954 @default.
• W2056098585 hasAuthorship W2056098585A5085243647 @default.
• W2056098585 hasAuthorship W2056098585A5086304089 @default.
• W2056098585 hasBestOaLocation W20560985851 @default.
• W2056098585 hasConcept C104317684 @default.
• W2056098585 hasConcept C138626823 @default.
• W2056098585 hasConcept C153911025 @default.
• W2056098585 hasConcept C159654299 @default.
• W2056098585 hasConcept C190062978 @default.
• W2056098585 hasConcept C190283241 @default.
• W2056098585 hasConcept C203014093 @default.
• W2056098585 hasConcept C2775934118 @default.
• W2056098585 hasConcept C2778236600 @default.
• W2056098585 hasConcept C28859421 @default.
• W2056098585 hasConcept C29311851 @default.
• W2056098585 hasConcept C31573885 @default.
• W2056098585 hasConcept C553184892 @default.
• W2056098585 hasConcept C55493867 @default.
• W2056098585 hasConcept C86803240 @default.
• W2056098585 hasConcept C95444343 @default.
• W2056098585 hasConceptScore W2056098585C104317684 @default.
• W2056098585 hasConceptScore W2056098585C138626823 @default.
• W2056098585 hasConceptScore W2056098585C153911025 @default.
• W2056098585 hasConceptScore W2056098585C159654299 @default.
• W2056098585 hasConceptScore W2056098585C190062978 @default.
• W2056098585 hasConceptScore W2056098585C190283241 @default.
• W2056098585 hasConceptScore W2056098585C203014093 @default.
• W2056098585 hasConceptScore W2056098585C2775934118 @default.
• W2056098585 hasConceptScore W2056098585C2778236600 @default.
• W2056098585 hasConceptScore W2056098585C28859421 @default.
• W2056098585 hasConceptScore W2056098585C29311851 @default.
• W2056098585 hasConceptScore W2056098585C31573885 @default.
• W2056098585 hasConceptScore W2056098585C553184892 @default.
• W2056098585 hasConceptScore W2056098585C55493867 @default.
• W2056098585 hasConceptScore W2056098585C86803240 @default.
• W2056098585 hasConceptScore W2056098585C95444343 @default.
• W2056098585 hasIssue "2" @default.
• W2056098585 hasLocation W20560985851 @default.
• W2056098585 hasLocation W20560985852 @default.
• W2056098585 hasOpenAccess W2056098585 @default.
• W2056098585 hasPrimaryLocation W20560985851 @default.
• W2056098585 hasRelatedWork W1969012488 @default.
• W2056098585 hasRelatedWork W2052760433 @default.
• W2056098585 hasRelatedWork W2055930187 @default.
• W2056098585 hasRelatedWork W2068903944 @default.
• W2056098585 hasRelatedWork W2086424522 @default.
• W2056098585 hasRelatedWork W2418290521 @default.
• W2056098585 hasRelatedWork W2473737552 @default.

• next