Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056116586> ?p ?o ?g. }
- W2056116586 endingPage "e97996" @default.
- W2056116586 startingPage "e97996" @default.
- W2056116586 abstract "In the biosynthesis of polyketides, ketoreductases (KRs) are an important group of enzymes that determine the chiralities of the carbon backbones. SiaM is a special member of this group that can recognize substrates with different lengths and can be used iteratively. Here we report the crystal structure of SiaM. Structural analysis indicates that the overall structure resembles those of other KRs. However, significant disparity can be found in the conserved LDD motif that is replaced with IRD motif in SiaM. The isoleucine and aspartic acid residues take similar orientations as leucine and aspartic acid in the conserved LDD motif, while the arginine residue points out towards the solvent. PISA analysis shows that SiaM forms a tetramer. Several aromatic residues are found in the interfaces, which have aromatic stacking interactions with the aromatic residues in the neighboring protomers. Mutagenesis studies performed on the aromatic residues show that these sites are important for maintaining the structural integrity of SiaM. However, the aromatic residues contribute differently to the enzymatic activity. In the N-terminal interface, the aromatic residues can be replaced with leucine without affecting the enzymatic activity while, in the other interface, such mutations abolish the enzymatic activity." @default.
- W2056116586 created "2016-06-24" @default.
- W2056116586 creator A5004117781 @default.
- W2056116586 creator A5010548726 @default.
- W2056116586 creator A5036532745 @default.
- W2056116586 creator A5048048291 @default.
- W2056116586 creator A5053621756 @default.
- W2056116586 creator A5053725057 @default.
- W2056116586 creator A5075679543 @default.
- W2056116586 creator A5090582248 @default.
- W2056116586 date "2014-06-05" @default.
- W2056116586 modified "2023-10-17" @default.
- W2056116586 title "Structural Insight into the Tetramerization of an Iterative Ketoreductase SiaM through Aromatic Residues in the Interfaces" @default.
- W2056116586 cites W1965404937 @default.
- W2056116586 cites W1967572086 @default.
- W2056116586 cites W1969153582 @default.
- W2056116586 cites W1970954035 @default.
- W2056116586 cites W1977038370 @default.
- W2056116586 cites W1978765166 @default.
- W2056116586 cites W1980075192 @default.
- W2056116586 cites W1982126789 @default.
- W2056116586 cites W1988530421 @default.
- W2056116586 cites W1988750758 @default.
- W2056116586 cites W1992724663 @default.
- W2056116586 cites W1994361647 @default.
- W2056116586 cites W1997275034 @default.
- W2056116586 cites W2008733674 @default.
- W2056116586 cites W2009254401 @default.
- W2056116586 cites W2025677032 @default.
- W2056116586 cites W2025888484 @default.
- W2056116586 cites W2026046929 @default.
- W2056116586 cites W2026058848 @default.
- W2056116586 cites W2026395206 @default.
- W2056116586 cites W2026943361 @default.
- W2056116586 cites W2028928748 @default.
- W2056116586 cites W2032538728 @default.
- W2056116586 cites W2033490251 @default.
- W2056116586 cites W2035503835 @default.
- W2056116586 cites W2040808395 @default.
- W2056116586 cites W2040909391 @default.
- W2056116586 cites W2041933909 @default.
- W2056116586 cites W2043532045 @default.
- W2056116586 cites W2045045577 @default.
- W2056116586 cites W2047941302 @default.
- W2056116586 cites W2050136339 @default.
- W2056116586 cites W2053914556 @default.
- W2056116586 cites W2059101972 @default.
- W2056116586 cites W2061190458 @default.
- W2056116586 cites W2062905154 @default.
- W2056116586 cites W2066088601 @default.
- W2056116586 cites W2073431498 @default.
- W2056116586 cites W2079178286 @default.
- W2056116586 cites W2080901247 @default.
- W2056116586 cites W2089237779 @default.
- W2056116586 cites W2097118224 @default.
- W2056116586 cites W2100063740 @default.
- W2056116586 cites W2108921801 @default.
- W2056116586 cites W2110442066 @default.
- W2056116586 cites W2117113877 @default.
- W2056116586 cites W2124026197 @default.
- W2056116586 cites W2143697213 @default.
- W2056116586 cites W2154714625 @default.
- W2056116586 cites W2160027326 @default.
- W2056116586 cites W2163341755 @default.
- W2056116586 cites W2180229411 @default.
- W2056116586 cites W4248872320 @default.
- W2056116586 doi "https://doi.org/10.1371/journal.pone.0097996" @default.
- W2056116586 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4046962" @default.
- W2056116586 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24901639" @default.
- W2056116586 hasPublicationYear "2014" @default.
- W2056116586 type Work @default.
- W2056116586 sameAs 2056116586 @default.
- W2056116586 citedByCount "4" @default.
- W2056116586 countsByYear W20561165862016 @default.
- W2056116586 countsByYear W20561165862017 @default.
- W2056116586 countsByYear W20561165862019 @default.
- W2056116586 countsByYear W20561165862022 @default.
- W2056116586 crossrefType "journal-article" @default.
- W2056116586 hasAuthorship W2056116586A5004117781 @default.
- W2056116586 hasAuthorship W2056116586A5010548726 @default.
- W2056116586 hasAuthorship W2056116586A5036532745 @default.
- W2056116586 hasAuthorship W2056116586A5048048291 @default.
- W2056116586 hasAuthorship W2056116586A5053621756 @default.
- W2056116586 hasAuthorship W2056116586A5053725057 @default.
- W2056116586 hasAuthorship W2056116586A5075679543 @default.
- W2056116586 hasAuthorship W2056116586A5090582248 @default.
- W2056116586 hasBestOaLocation W20561165861 @default.
- W2056116586 hasConcept C132677234 @default.
- W2056116586 hasConcept C147816474 @default.
- W2056116586 hasConcept C178790620 @default.
- W2056116586 hasConcept C181199279 @default.
- W2056116586 hasConcept C185592680 @default.
- W2056116586 hasConcept C2776580952 @default.
- W2056116586 hasConcept C2777305461 @default.
- W2056116586 hasConcept C2777992278 @default.
- W2056116586 hasConcept C2778886173 @default.
- W2056116586 hasConcept C2781208047 @default.
- W2056116586 hasConcept C2781338088 @default.