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• W2056154328 abstract "The sonicated and fractionated poly(rG)·poly(rC) (I) and poly(dG)·poly(dC) (II) samples mostly with Na+ counterions were studied, on a comparative basis, to determine the spatial arrangement of the constituent G and C bases with respect to the double-stranded helical axis in ionic aqueous solutions. The reduced electric linear dichroism, Δε/ε, was measured at 7 °C in 1.5−24 kV/cm field strength and 300−190 nm wavelength regions. By analyzing the field strength dependence of observed Δε/ε values with theoretical orientation functions, the saturated reduced dichroism, (Δε/ε)s, at 260 nm was evaluated to be −0.96 ± 0.02 for I and −0.93 ± 0.01 for II. The electric linear dichroism (ELD) spectra of these duplexes are very close to each other, being undulatory, but not constant, in the whole wavelength region. The (Δε/ε)s values are never as high as −1.5, indicating that the optical transition moments of the nucleic acid bases responsible for the UV absorption bands are not normal to the helix axis. By analyzing the ELD spectra with a newly developed technique, the inclination angle α, the roll angle θR, and the tilt angle θT were evaluated for the individual bases. The most likely values are 24°, 1°, and 24° for rG; 26°, 1°, and 26° for rC in the rG·rC base pair; 26°, 2°, and 26° for dG; 29°, 3°, and 29° for dC in the dG·dC pair. Thus, the base pairs are only slightly bent and propeller twisted, being nearly coplanar. Both duplexes were concluded to exist in the same conformation and to belong to the same A-form family in solutions in spite of the different sugar structure, contrary to X-ray results in the solid state." @default.
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• W2056154328 title "A Comparative Study on the Structure of Double-Stranded Antiparallel Poly(riboguanylic acid)·Poly(ribocytidylic acid) and Poly(deoxyriboguanylic acid)·Poly(deoxyribocytidylic acid) Helices in Solution by Pulsed Electric Linear Dichroism" @default.
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• W2056154328 doi "https://doi.org/10.1021/jp962590m" @default.
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