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- W2056193211 abstract "A high density culture method was devised to improve the yield of berberine from highly productive cells of Coptis japonica. By adjusting aeration and stirring, Coptis cells were cultured at densities of up to 75 g dm−3 (dry weight) in a culture tank fitted with a hollow-paddle type stirrer. Whereas a maximum density of 30 g dm−3 of C. japonica cells could be used in ordinary batch culture, 48 g dm−3 could be used in a fed-batch culture in which the amounts of the nutrients in the medium were made proportional to the density of the inoculum. Moreover, in fed-batch culture done with modified medium, the composition of which had been determined from the amounts of components incorporated in cells grown at the usual density for ordinary batch culture, the cell yield was improved to 55 g dm−3 and the berberine yield to 3.5 g dm−3." @default.
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- W2056193211 date "2007-04-24" @default.
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- W2056193211 title "High density culture of Coptis japonica cells increases berberine production" @default.
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- W2056193211 doi "https://doi.org/10.1002/jctb.280460107" @default.
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