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- W2056223887 abstract "Protoplasts isolated from callus tissue derived from freshly cultured stem segments of Tylophora indica (Asclepiadaceae) rapidly divided and resulted in callus. Subsequent embryoid/ shoot bud differentiation in callus was observed on MS medium supplemented with auxins and cytokinins. The embryoids/shoot buds on transfer to appropriate basal medium without growth substances grew into plantlets. Protoplasts isolated from callus tissue maintained for over five years showed divisions and subsequent callus formation but failed to regenerate plants." @default.
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- W2056223887 date "1984-07-01" @default.
- W2056223887 modified "2023-09-27" @default.
- W2056223887 title "Plant Regeneration in Protoplast Cultures of Tylophora indica" @default.
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- W2056223887 doi "https://doi.org/10.1016/s0176-1617(84)80125-x" @default.
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