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• W2056267788 abstract "Cis-regulatory DNA sequences causally mediate patterns of gene expression, but efficient experimental analysis of these control systems has remained challenging. Here we develop a new version of “barcoded DNA-tag reporters, “Nanotags that permit simultaneous quantitative analysis of up to 130 distinct cis-regulatory modules (CRMs). The activities of these reporters are measured in single experiments by the NanoString RNA counting method and other quantitative procedures. We demonstrate the efficiency of the Nanotag method by simultaneously measuring hourly temporal activities of 126 CRMs from 46 genes in the developing sea urchin embryo, otherwise a virtually impossible task. Nanotags are also used in gene perturbation experiments to reveal cis-regulatory responses of many CRMs at once. Nanotag methodology can be applied to many research areas, ranging from gene regulatory networks to functional and evolutionary genomics." @default.
• W2056267788 created "2016-06-24" @default.
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• W2056267788 date "2012-04-26" @default.
• W2056267788 modified "2023-09-23" @default.
• W2056267788 title "Barcoded DNA-Tag Reporters for Multiplex Cis-Regulatory Analysis" @default.
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• W2056267788 doi "https://doi.org/10.1371/journal.pone.0035934" @default.
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