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- W2056291285 abstract "Mouse monoclonal antibodies (MAbs) and mouse and rabbit (polyclonal) antisera were raised against a Trichoderma harzianum isolate T95. A cell line, Th.HD33, raised from splenocytes from a mouse immunized with lyophilized mycelium and Quil A adjuvant, produced MAbs of the immunoglobulin sub-class IgG2a that were specific recognizing, by ELISA, antigens from a number of Trichoderma species and species from the related genus Hypocrea but not from a range of other soil-borne fungi. These MAbs were used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of live propagules of Trichoderma harzianum in a peat-bran medium. Heat, protease and periodate treatment of the T95 antigen and binding on Western blots indicate that the Th.HD33 MAbs recognized a protein epitope of ca Mr 18,500 which forms part of a larger glycoprotein molecule." @default.
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- W2056291285 date "1994-07-01" @default.
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- W2056291285 title "Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of live propagules of Trichoderma harzianum in a peat-bran medium" @default.
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- W2056291285 doi "https://doi.org/10.1016/0038-0717(94)90307-7" @default.
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