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• W2056307424 endingPage "6400" @default.
• W2056307424 startingPage "6394" @default.
• W2056307424 abstract "ABSTRACT Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5′ end, which are deleted during replication to generate authentic viral genomes. We removed those 5′-end extra nucleotides utilizing a hammerhead ribozyme to produce transcripts with accurate 5′ ends. These transcripts replicate substantially more rapidly in cell culture, demonstrating no lag before replication; they also replicate more efficiently in Xenopus laevis oocytes and in in vitro translation-replication cell extracts. In both systems, an exact 5′ end is necessary for synthesis of positive-strand RNA but not negative-strand RNA." @default.
• W2056307424 created "2016-06-24" @default.
• W2056307424 creator A5006463024 @default.
• W2056307424 creator A5030357593 @default.
• W2056307424 date "2000-07-15" @default.
• W2056307424 modified "2023-09-25" @default.
• W2056307424 title "Poliovirus Requires a Precise 5′ End for Efficient Positive-Strand RNA Synthesis" @default.
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• W2056307424 doi "https://doi.org/10.1128/jvi.74.14.6394-6400.2000" @default.
• W2056307424 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/112146" @default.
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