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- W2056340890 abstract "Two cDNA clones for complement component C2 have been isolated from a high-complexity human liver cDNA library by using a mixture of 64 synthetic oligonucleotides as a probe. The 400-base-pair insert of pC201 codes for a region containing the active site serine residue and the secondary substrate binding pocket of the serine protease. This part of C2 is 34% homologous to the corresponding region of the related serine protease factor B and additional similarity is evident from a number of conservative amino acid replacements in this region. The insert of pC201 was used as a specific probe in RNA transfer analysis to determine the size of the C2 mRNA as approximately equal to 2.9 kilobases. Southern blot analysis of genomic DNA of unrelated individuals identified a single C2 locus and showed no cross-hybridization with the factor B locus." @default.
- W2056340890 created "2016-06-24" @default.
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- W2056340890 date "1984-02-01" @default.
- W2056340890 modified "2023-09-26" @default.
- W2056340890 title "Isolation of cDNA clones for human complement component C2." @default.
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- W2056340890 doi "https://doi.org/10.1073/pnas.81.4.1212" @default.
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