FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056341520> ?p ?o ?g. }

• W2056341520 endingPage "e7946" @default.
• W2056341520 startingPage "e7946" @default.
• W2056341520 abstract "To initiate infection, Bacillus anthracis needs to overcome the host innate immune system. Anthrax toxin, a major virulence factor of B. anthracis, impairs both the innate and adaptive immune systems and is important in the establishment of anthrax infections.To measure the ability of anthrax toxin to target immune cells, studies were performed using a fusion of the anthrax toxin lethal factor (LF) N-terminal domain (LFn, aa 1-254) with beta-lactamase (LFnBLA). This protein reports on the ability of the anthrax toxin protective antigen (PA) to mediate LF delivery into cells. Primary immune cells prepared from mouse spleens were used in conjunction with flow cytometry to assess cleavage and resulting FRET disruption of a fluorescent beta-lactamase substrate, CCF2/AM. In spleen cell suspensions, the macrophages, dendritic cells, and B cells showed about 75% FRET disruption of CCF2/AM due to cleavage by the PA-delivered LFnBLA. LFnBLA delivery into CD4+ and CD8+ T cells was lower, with 40% FRET disruption. When the analyses were done on purified samples of individual cell types, similar results were obtained, with T cells again having lower LFnBLA delivery than macrophages, dendritic cells, and B cells. Relative expression levels of the toxin receptors CMG2 and TEM8 on these cells were determined by real-time PCR. Expression of CMG2 was about 1.5-fold higher in CD8+ cells than in CD4+ and B cells, and 2.5-fold higher than in macrophages.Anthrax toxin entry and activity differs among immune cells. Macrophages, dendritic cells, and B cells displayed higher LFnBLA activity than CD4+ and CD8+ T cells in both spleen cell suspension and the purified samples of individual cell types. Expression of anthrax toxin receptor CMG2 is higher in CD4+ and CD8+ T cells, which is not correlated to the intracellular LFnBLA activity." @default.
• W2056341520 created "2016-06-24" @default.
• W2056341520 creator A5043746736 @default.
• W2056341520 creator A5086376220 @default.
• W2056341520 date "2009-11-23" @default.
• W2056341520 modified "2023-10-03" @default.
• W2056341520 title "Anthrax Toxin Uptake by Primary Immune Cells as Determined with a Lethal Factor-β-Lactamase Fusion Protein" @default.
• W2056341520 cites W125745120 @default.
• W2056341520 cites W1559668074 @default.
• W2056341520 cites W1872704179 @default.
• W2056341520 cites W1914152834 @default.
• W2056341520 cites W1964366578 @default.
• W2056341520 cites W1968770640 @default.
• W2056341520 cites W1975648878 @default.
• W2056341520 cites W1978789596 @default.
• W2056341520 cites W1980066894 @default.
• W2056341520 cites W1995910871 @default.
• W2056341520 cites W2004673387 @default.
• W2056341520 cites W2004693684 @default.
• W2056341520 cites W2015148410 @default.
• W2056341520 cites W2025395578 @default.
• W2056341520 cites W2049236196 @default.
• W2056341520 cites W2053168741 @default.
• W2056341520 cites W2063847765 @default.
• W2056341520 cites W2065278363 @default.
• W2056341520 cites W2068309990 @default.
• W2056341520 cites W2083670089 @default.
• W2056341520 cites W2085414634 @default.
• W2056341520 cites W2091577374 @default.
• W2056341520 cites W2093329456 @default.
• W2056341520 cites W2099471869 @default.
• W2056341520 cites W2100249681 @default.
• W2056341520 cites W2102995091 @default.
• W2056341520 cites W2109074284 @default.
• W2056341520 cites W2111794178 @default.
• W2056341520 cites W2124919081 @default.
• W2056341520 cites W2128855764 @default.
• W2056341520 cites W2133013758 @default.
• W2056341520 cites W2143792029 @default.
• W2056341520 cites W2145293058 @default.
• W2056341520 cites W2145742637 @default.
• W2056341520 cites W2153738223 @default.
• W2056341520 cites W2156236359 @default.
• W2056341520 cites W2157970796 @default.
• W2056341520 cites W2162830222 @default.
• W2056341520 cites W2163083344 @default.
• W2056341520 cites W2171740144 @default.
• W2056341520 cites W67016306 @default.
• W2056341520 cites W2622429885 @default.
• W2056341520 doi "https://doi.org/10.1371/journal.pone.0007946" @default.
• W2056341520 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2775957" @default.
• W2056341520 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19956758" @default.
• W2056341520 hasPublicationYear "2009" @default.
• W2056341520 type Work @default.
• W2056341520 sameAs 2056341520 @default.
• W2056341520 citedByCount "26" @default.
• W2056341520 countsByYear W20563415202012 @default.
• W2056341520 countsByYear W20563415202013 @default.
• W2056341520 countsByYear W20563415202014 @default.
• W2056341520 countsByYear W20563415202015 @default.
• W2056341520 countsByYear W20563415202016 @default.
• W2056341520 countsByYear W20563415202017 @default.
• W2056341520 countsByYear W20563415202019 @default.
• W2056341520 countsByYear W20563415202020 @default.
• W2056341520 countsByYear W20563415202021 @default.
• W2056341520 countsByYear W20563415202022 @default.
• W2056341520 crossrefType "journal-article" @default.
• W2056341520 hasAuthorship W2056341520A5043746736 @default.
• W2056341520 hasAuthorship W2056341520A5086376220 @default.
• W2056341520 hasBestOaLocation W20563415201 @default.
• W2056341520 hasConcept C104317684 @default.
• W2056341520 hasConcept C123894998 @default.
• W2056341520 hasConcept C136449434 @default.
• W2056341520 hasConcept C153911025 @default.
• W2056341520 hasConcept C167508917 @default.
• W2056341520 hasConcept C203014093 @default.
• W2056341520 hasConcept C2777289321 @default.
• W2056341520 hasConcept C2777367657 @default.
• W2056341520 hasConcept C2780239966 @default.
• W2056341520 hasConcept C2780801004 @default.
• W2056341520 hasConcept C2781090273 @default.
• W2056341520 hasConcept C40767141 @default.
• W2056341520 hasConcept C41810244 @default.
• W2056341520 hasConcept C523546767 @default.
• W2056341520 hasConcept C54355233 @default.
• W2056341520 hasConcept C553184892 @default.
• W2056341520 hasConcept C55493867 @default.
• W2056341520 hasConcept C60987743 @default.
• W2056341520 hasConcept C86803240 @default.
• W2056341520 hasConcept C8891405 @default.
• W2056341520 hasConcept C89423630 @default.
• W2056341520 hasConceptScore W2056341520C104317684 @default.
• W2056341520 hasConceptScore W2056341520C123894998 @default.
• W2056341520 hasConceptScore W2056341520C136449434 @default.
• W2056341520 hasConceptScore W2056341520C153911025 @default.
• W2056341520 hasConceptScore W2056341520C167508917 @default.
• W2056341520 hasConceptScore W2056341520C203014093 @default.
• W2056341520 hasConceptScore W2056341520C2777289321 @default.

• next