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• W2056423224 abstract "The laboratory criteria for the antiphospholipid syndrome (APS) include lupus anticoagulants (LAC), medium or high titer (>40 GPL or MPL, or >99th percentile) anticardiolipin antibodies (aCL) or anti-β2-glycoprotein I antibodies (aβ2GPI) (>99th percentile) [ [1] Miyakis S. Lockshin M.D. Atsumi T. Branch D.W. Brey R.L. Cervera R. et al. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost. Feb. 2006; 4: 295-306 Crossref PubMed Scopus (4991) Google Scholar ]. aCL and aβ2GPI should be measured by standardized enzyme-linked immunosorbent assays (ELISA) following recommended procedures [ 2 Wong R.C. Consensus guidelines for anticardiolipin antibody testing. Thromb Res. 2004; 114: 559-571 Abstract Full Text Full Text PDF PubMed Scopus (41) Google Scholar , 3 Reber G. Tincani A. Sanmarco M. de Moerloose P. Boffa M.C. Proposals for the measurement of anti-beta2-glycoprotein I antibodies. Standardization group of the European Forum on Antiphospholipid Antibodies. J Thromb Haemost. Oct. 2004; 2: 1860-1862 Crossref PubMed Scopus (136) Google Scholar ]. Coagulation assays for LAC as well as ELISAs for the detection of antiphospholipid antibodies antibodies (aPL) show methodological shortcomings [ 4 Devreese K. Hoylaerts M.F. Laboratory diagnosis of the antiphospholipid syndrome: a plethora of obstacles to overcome. Eur J Haematol. Jul. 2009; 83: 1-16 Crossref PubMed Scopus (72) Google Scholar , 5 Urbanus R.T. de Groot P.G. Antiphospholipid antibodies–we are not quite there yet. Blood Rev. Mar. 2011; 25: 97-106 Abstract Full Text Full Text PDF PubMed Scopus (39) Google Scholar ]. In spite of tremendous efforts, standardization has not been achieved and continues to be a topic of discussion and concern to scientists, laboratory workers and clinicians; especially for the solid phase assays, since progress has been made in the detection of LAC thanks to the updated guidelines of 2009 [ [6] Pengo V. Tripodi A. Reber G. Rand J.H. Ortel T.L. Galli M. et al. Update of the guidelines for lupus anticoagulant detection. Subcommittee on Lupus Anticoagulant/Antiphospholipid Antibody of the Scientific and Standardisation Committee of the International Society on Thrombosis and Haemostasis. J Thromb Haemost. Oct. 2009; 7: 1737-1740 Crossref PubMed Scopus (946) Google Scholar ]." @default.
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• W2056423224 title "Anticardiolipin and anti-β2glycoprotein-I antibody cut-off values in the diagnosis of antiphospholipid syndrome: more than calculating the in-house 99th percentiles, even for new automated assays" @default.
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• W2056423224 doi "https://doi.org/10.1016/j.thromres.2011.06.023" @default.
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