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- W2056510901 abstract "An in vitro protocol was developed to screen Coffea arabica genotypes for resistance to coffee berry disease caused by Colletotrichum kahawae. Initially, cultural conditions which influenced the growth of isolates of C. kahawae on agar media suitable for callus growth were determined. The growth of the fungus on the callus derived from susceptible and resistant genotypes was then assessed. This ensured that no detrimental competition for nutrients between the pathogen and the calli occurred. Optimisation of the concentration of the phytohormones added to the media, the temperature and incubation period were found to be important in the expression of differential responses of calli to inoculation with the pathogen as detected by measurement of hyphal growth. The screening of calli of nine C. arabica genotypes showed that this method identified genotypes highly resistant or susceptible to the disease and was sufficiently sensitive to distinguish those genotypes with moderate or low resistance." @default.
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- W2056510901 date "1995-10-01" @default.
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- W2056510901 title "In vitro screening of coffee genotypes for resistance to coffee berry disease (Colletotrichum kahawae)" @default.
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- W2056510901 doi "https://doi.org/10.1111/j.1744-7348.1995.tb06670.x" @default.
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