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- W2056512830 abstract "CRISPR-Cas9 can be used to edit both single and multiple genes in postmitotic neurons in adult mice enabling rapid assessment of gene functions in the brain. Probing gene function in the mammalian brain can be greatly assisted with methods to manipulate the genome of neurons in vivo. The clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease (Cas)9 from Streptococcus pyogenes (SpCas9)1 can be used to edit single or multiple genes in replicating eukaryotic cells, resulting in frame-shifting insertion/deletion (indel) mutations and subsequent protein depletion. Here, we delivered SpCas9 and guide RNAs using adeno-associated viral (AAV) vectors to target single (Mecp2) as well as multiple genes (Dnmt1, Dnmt3a and Dnmt3b) in the adult mouse brain in vivo. We characterized the effects of genome modifications in postmitotic neurons using biochemical, genetic, electrophysiological and behavioral readouts. Our results demonstrate that AAV-mediated SpCas9 genome editing can enable reverse genetic studies of gene function in the brain." @default.
- W2056512830 created "2016-06-24" @default.
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- W2056512830 date "2014-10-19" @default.
- W2056512830 modified "2023-10-11" @default.
- W2056512830 title "In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9" @default.
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- W2056512830 doi "https://doi.org/10.1038/nbt.3055" @default.
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