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- W2056514513 abstract "Fluorescence microscopy has become a powerful and standard complementary technique in the study of amphiphilic films at the air-water interface. For nearly three decades the coupling of traditional thermodynamic measurements with direct visualization has provided a better understanding of self-assembled Langmuir monolayers and their application in the study of the physical properties of membranes and interfaces. As an introduction we provide a brief overview of this established technique and demonstrate its continued utility in the recent observation of novel phase behavior in monolayers of 25-hydroxycholesterol (25-OH) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). We then focus our review on new analysis techniques which take advantage of the ability to store, process, and analyze large sets of images. We pay particular attention to efforts measuring the line tension between coexisting two dimensional fluid phases in the Langmuir monolayer. Using non-perturbative methods, we can measure fundamental mechanical properties of these two dimensional systems. Finally, we highlight the use of Model Convolution Microscopy as a new tool to provide insight on the experimental limits in these studies." @default.
- W2056514513 created "2016-06-24" @default.
- W2056514513 creator A5029978651 @default.
- W2056514513 creator A5059549535 @default.
- W2056514513 creator A5064796076 @default.
- W2056514513 date "2010-07-01" @default.
- W2056514513 modified "2023-09-26" @default.
- W2056514513 title "Taking another look with fluorescence microscopy: Image processing techniques in Langmuir monolayers for the twenty-first century" @default.
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