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- W2056516429 abstract "The morphological and membrane phenotypic characteristics of normal peripheral blood B-lymphocytes were studied at ultrastructural level by means of the immunogold method with the following McAb : anti-HLA-Dr (OKIa and FMC4), FMC7, OKT10, and αHC1 and αHC2, reactive with HCL cells. Five morphological subsets of B-lymphocytes were identified: (i) 40% had a high nuclear cytoplasmic ratio, well condensed chromatin and small pale granules localised in one area of the cytoplasm, these cells expressed HLA-Dr and did not react with any of the other McAb; (ii) 40% had a prominent nucleolus, variable amount of chromatin condensation and localised granules, these cells were HLA-Dr positive and some of them expressed FMC7 as do the cells of B-PLL; (iii) 10% had a villous outline and variable numbers of polyribosomes scattered throughout the cytoplasm, some of these cells resembled morphologically HCL cells and were HLA-Dr+, FMC7+, αHC1+, αHC2+ and OKT10+/−. The remaining two types of B-cells (10%) had the morphology of antibody secreting cells with parallel arrays of endoplasmic reticulum and some displayed electron dense granules in the Golgi zone. These cells were: (iv) lymphoplasmacytoid, the more common type, which were HLA-Dr+/−, FMC7−, αHC1−, αHC2+ and OKT10+/−, and (v) plasma cells which were positive only with OKT10. The similarities in morphology and membrane markers between two of the subsets defined in this study and the cells of B-PLL and HCL suggest that they may represent normal counterparts of these B-cell malignancies." @default.
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- W2056516429 date "1985-01-01" @default.
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- W2056516429 title "Normal counterparts of hairy cells and B-prolymphocytes in the peripheral blood. An ultrastructural study with monoclonal antibodies and the immunogold method" @default.
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- W2056516429 doi "https://doi.org/10.1016/0145-2126(85)90055-4" @default.
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