FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056520296> ?p ?o ?g. }

• W2056520296 endingPage "198" @default.
• W2056520296 startingPage "194" @default.
• W2056520296 abstract "In this paper, we report a novel method for delivering genes into chloroplasts of tobacco cells using laser microablation. The plasmid pLD200-GFP was introduced into chloroplasts of Nicotiana tabacum cv. Xanthi guard cells and transient GFP expression was detected in the chloroplasts after 2-3 d of incubation. The technique uses an argon fluoride (ArF) excimer laser to perforate the cell surface in a 4 mum(2) area in the presence of plasmid coated gold microparticles. Pretreatment of guard cells to promote stomatal closure prior to laser ablation resulted in a significant increase in the survival rate of cells and a transient expression rate of 2-3% in trial number basis was archived. Our method has unique advantages such as avoiding laborious pretreatments that adversely affect cell viability and specific delivery of transgenes into a desired cell in complex leaf tissue. This technique is a potential tool for cell specific transient gene expression studies for elucidation of gene regulation and expression." @default.
• W2056520296 created "2016-06-24" @default.
• W2056520296 creator A5015605291 @default.
• W2056520296 creator A5037814947 @default.
• W2056520296 creator A5066301304 @default.
• W2056520296 creator A5068089899 @default.
• W2056520296 creator A5068540369 @default.
• W2056520296 creator A5068906179 @default.
• W2056520296 creator A5079042022 @default.
• W2056520296 date "2008-08-01" @default.
• W2056520296 modified "2023-10-03" @default.
• W2056520296 title "Transient gene expression in guard cell chloroplasts of tobacco using ArF excimer laser microablation" @default.
• W2056520296 cites W1506710402 @default.
• W2056520296 cites W1559267289 @default.
• W2056520296 cites W1977537416 @default.
• W2056520296 cites W1983251735 @default.
• W2056520296 cites W2002829704 @default.
• W2056520296 cites W2005412677 @default.
• W2056520296 cites W2020986744 @default.
• W2056520296 cites W2026311140 @default.
• W2056520296 cites W2032379550 @default.
• W2056520296 cites W2033975186 @default.
• W2056520296 cites W2040679387 @default.
• W2056520296 cites W2049160993 @default.
• W2056520296 cites W2066811776 @default.
• W2056520296 cites W2085983043 @default.
• W2056520296 cites W2089934847 @default.
• W2056520296 cites W2092164769 @default.
• W2056520296 cites W2109276758 @default.
• W2056520296 cites W2114265434 @default.
• W2056520296 cites W2131491171 @default.
• W2056520296 cites W2138643584 @default.
• W2056520296 doi "https://doi.org/10.1263/jbb.106.194" @default.
• W2056520296 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18804064" @default.
• W2056520296 hasPublicationYear "2008" @default.
• W2056520296 type Work @default.
• W2056520296 sameAs 2056520296 @default.
• W2056520296 citedByCount "7" @default.
• W2056520296 countsByYear W20565202962012 @default.
• W2056520296 countsByYear W20565202962013 @default.
• W2056520296 countsByYear W20565202962015 @default.
• W2056520296 countsByYear W20565202962022 @default.
• W2056520296 crossrefType "journal-article" @default.
• W2056520296 hasAuthorship W2056520296A5015605291 @default.
• W2056520296 hasAuthorship W2056520296A5037814947 @default.
• W2056520296 hasAuthorship W2056520296A5066301304 @default.
• W2056520296 hasAuthorship W2056520296A5068089899 @default.
• W2056520296 hasAuthorship W2056520296A5068540369 @default.
• W2056520296 hasAuthorship W2056520296A5068906179 @default.
• W2056520296 hasAuthorship W2056520296A5079042022 @default.
• W2056520296 hasConcept C102230213 @default.
• W2056520296 hasConcept C104317684 @default.
• W2056520296 hasConcept C120665830 @default.
• W2056520296 hasConcept C121332964 @default.
• W2056520296 hasConcept C12554922 @default.
• W2056520296 hasConcept C136547172 @default.
• W2056520296 hasConcept C142613039 @default.
• W2056520296 hasConcept C1491633281 @default.
• W2056520296 hasConcept C150194340 @default.
• W2056520296 hasConcept C153911025 @default.
• W2056520296 hasConcept C185592680 @default.
• W2056520296 hasConcept C2780477314 @default.
• W2056520296 hasConcept C2781205006 @default.
• W2056520296 hasConcept C520434653 @default.
• W2056520296 hasConcept C55493867 @default.
• W2056520296 hasConcept C69305403 @default.
• W2056520296 hasConcept C86803240 @default.
• W2056520296 hasConcept C95444343 @default.
• W2056520296 hasConceptScore W2056520296C102230213 @default.
• W2056520296 hasConceptScore W2056520296C104317684 @default.
• W2056520296 hasConceptScore W2056520296C120665830 @default.
• W2056520296 hasConceptScore W2056520296C121332964 @default.
• W2056520296 hasConceptScore W2056520296C12554922 @default.
• W2056520296 hasConceptScore W2056520296C136547172 @default.
• W2056520296 hasConceptScore W2056520296C142613039 @default.
• W2056520296 hasConceptScore W2056520296C1491633281 @default.
• W2056520296 hasConceptScore W2056520296C150194340 @default.
• W2056520296 hasConceptScore W2056520296C153911025 @default.
• W2056520296 hasConceptScore W2056520296C185592680 @default.
• W2056520296 hasConceptScore W2056520296C2780477314 @default.
• W2056520296 hasConceptScore W2056520296C2781205006 @default.
• W2056520296 hasConceptScore W2056520296C520434653 @default.
• W2056520296 hasConceptScore W2056520296C55493867 @default.
• W2056520296 hasConceptScore W2056520296C69305403 @default.
• W2056520296 hasConceptScore W2056520296C86803240 @default.
• W2056520296 hasConceptScore W2056520296C95444343 @default.
• W2056520296 hasIssue "2" @default.
• W2056520296 hasLocation W20565202961 @default.
• W2056520296 hasLocation W20565202962 @default.
• W2056520296 hasOpenAccess W2056520296 @default.
• W2056520296 hasPrimaryLocation W20565202961 @default.
• W2056520296 hasRelatedWork W1963661638 @default.
• W2056520296 hasRelatedWork W1979647093 @default.
• W2056520296 hasRelatedWork W2034380381 @default.
• W2056520296 hasRelatedWork W2037814110 @default.
• W2056520296 hasRelatedWork W2056520296 @default.
• W2056520296 hasRelatedWork W2070272049 @default.
• W2056520296 hasRelatedWork W2084575808 @default.

• next