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• W2056523314 abstract "ObjectiveNO is one of many reactive oxygen free species (ROS) associated with inflammation and preterm birth. Endogenous TAC neutralizes ROS normally maintaining an overall anti oxidant state. We have previously shown that the AF profile in women undergoing rescue cerclage is the same as those in idiopathic preterm labor where inflammation is prevalent. We tested the hypothesis that either AF TAC and/or NO correlate with the outcome of rescue cerclage.Study DesignCase-control study- 25 women with cervical dilatation (>2cm) and bulging membranes between 16 and 25 weeks gestation were treated by rescue cerclage. Amniocentesis was performed pre-cerclage to facilitate membrane reduction. An additional 55 women between 16 and 25 weeks who underwent amniocentesis for chromosomal test provided control samples. AF samples were tested for TAC and total NO, and then correlated with pregnancy outcome. TAC was measured using ABTS assay. Griess reagents were used to determine the NO concentration.ResultsAF TAC levels were significantly lower in women with CI than control group (CI, 1030 mmol/L vs. control, 1361 mmol/L, p=.01) suggesting increased consumption, while AF NO levels were similar (CI, 13.3 μM vs. control, 12.3 μM, p=.11). Low levels of AF TAC seemed to be associated with a shorter cerclage to delivery interval (R=0.1006, p=0.056). Pregnancy outcome of control group was better than CI group (p < .01).ConclusionsAF TAC seemed to be associated with a shorter cerclage to delivery intervals. NO levels are not different between cerclage and control groups which suggests NO is uninvolved in the obligatory cervical ripening. ObjectiveNO is one of many reactive oxygen free species (ROS) associated with inflammation and preterm birth. Endogenous TAC neutralizes ROS normally maintaining an overall anti oxidant state. We have previously shown that the AF profile in women undergoing rescue cerclage is the same as those in idiopathic preterm labor where inflammation is prevalent. We tested the hypothesis that either AF TAC and/or NO correlate with the outcome of rescue cerclage. NO is one of many reactive oxygen free species (ROS) associated with inflammation and preterm birth. Endogenous TAC neutralizes ROS normally maintaining an overall anti oxidant state. We have previously shown that the AF profile in women undergoing rescue cerclage is the same as those in idiopathic preterm labor where inflammation is prevalent. We tested the hypothesis that either AF TAC and/or NO correlate with the outcome of rescue cerclage. Study DesignCase-control study- 25 women with cervical dilatation (>2cm) and bulging membranes between 16 and 25 weeks gestation were treated by rescue cerclage. Amniocentesis was performed pre-cerclage to facilitate membrane reduction. An additional 55 women between 16 and 25 weeks who underwent amniocentesis for chromosomal test provided control samples. AF samples were tested for TAC and total NO, and then correlated with pregnancy outcome. TAC was measured using ABTS assay. Griess reagents were used to determine the NO concentration. Case-control study- 25 women with cervical dilatation (>2cm) and bulging membranes between 16 and 25 weeks gestation were treated by rescue cerclage. Amniocentesis was performed pre-cerclage to facilitate membrane reduction. An additional 55 women between 16 and 25 weeks who underwent amniocentesis for chromosomal test provided control samples. AF samples were tested for TAC and total NO, and then correlated with pregnancy outcome. TAC was measured using ABTS assay. Griess reagents were used to determine the NO concentration. ResultsAF TAC levels were significantly lower in women with CI than control group (CI, 1030 mmol/L vs. control, 1361 mmol/L, p=.01) suggesting increased consumption, while AF NO levels were similar (CI, 13.3 μM vs. control, 12.3 μM, p=.11). Low levels of AF TAC seemed to be associated with a shorter cerclage to delivery interval (R=0.1006, p=0.056). Pregnancy outcome of control group was better than CI group (p < .01). AF TAC levels were significantly lower in women with CI than control group (CI, 1030 mmol/L vs. control, 1361 mmol/L, p=.01) suggesting increased consumption, while AF NO levels were similar (CI, 13.3 μM vs. control, 12.3 μM, p=.11). Low levels of AF TAC seemed to be associated with a shorter cerclage to delivery interval (R=0.1006, p=0.056). Pregnancy outcome of control group was better than CI group (p < .01). ConclusionsAF TAC seemed to be associated with a shorter cerclage to delivery intervals. NO levels are not different between cerclage and control groups which suggests NO is uninvolved in the obligatory cervical ripening. AF TAC seemed to be associated with a shorter cerclage to delivery intervals. NO levels are not different between cerclage and control groups which suggests NO is uninvolved in the obligatory cervical ripening." @default.
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• W2056523314 date "2011-01-01" @default.
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• W2056523314 title "132: Role of amniotic fluid (AF) nitric oxide (NO) and total antioxidant capacity (TAC) on cervical incompetence (CI)" @default.
• W2056523314 doi "https://doi.org/10.1016/j.ajog.2010.10.148" @default.
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