FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056523976> ?p ?o ?g. }

• W2056523976 abstract "Previous studies have demonstrated that bradykinin stimulates the rapid release of inositol 1,4,5 trisphosphate (IP3) from membrane phosphatidylinositol 4,5 bisphosphate (PIP2) in Madin-Darby canine kidney (MDCK) cells. Since current evidence would suggest that the activation of phospholipase C (PLC) is mediated through a guanine nucleotide-binding protein in receptor-mediated activation of PLC, we evaluated the role of guanine nucleotide proteins in receptor-mediated (bradykinin-stimulated) activation of PLC in MDCK cells. Bradykinin at 10(-7) M produced a marked increase in IP3 formation within 10 s increasing from a basal level of 46.2 to 686.6 pmol/mg cell protein a 15-fold increase. Pretreatment of MDCK cells in culture with 200 ng/ml of pertussis toxin for 4 h reduced the bradykinin-stimulated response to 205.8 pmol/mg protein. A 41-kD protein substrate in MDCK membranes was ADP ribosylated in vitro in the presence of pertussis toxin. The ADP ribosylation in vitro was inhibited by pretreatment of the cells in culture with pertussis toxin. Membranes from MDCK cells incubated in the presence of [3H]PIP2/phosphatidyl ethanolamine liposomes demonstrated hydrolysis of [3H]PIP2 with release of [3H]IP3 when GTP 100 microM or GTP gamma S 10 microM was added. Bradykinin 10(-7) M added with GTP 100 microM markedly increased the rate of hydrolysis within 10 s, thus demonstrating a similar time course of PLC activation as intact cells. These results demonstrate that bradykinin binds to its receptor and activates a membrane-associated PLC through a pertussis toxin-sensitive, guanine nucleotide protein." @default.
• W2056523976 created "2016-06-24" @default.
• W2056523976 creator A5005094554 @default.
• W2056523976 creator A5040985491 @default.
• W2056523976 creator A5004837178 @default.
• W2056523976 date "1988-06-01" @default.
• W2056523976 modified "2023-10-16" @default.
• W2056523976 title "Bradykinin-activated membrane-associated phospholipase C in Madin-Darby canine kidney cells." @default.
• W2056523976 cites W147014518 @default.
• W2056523976 cites W1498162448 @default.
• W2056523976 cites W1503509391 @default.
• W2056523976 cites W1513905140 @default.
• W2056523976 cites W1521095349 @default.
• W2056523976 cites W1527459196 @default.
• W2056523976 cites W1528087101 @default.
• W2056523976 cites W1547665114 @default.
• W2056523976 cites W1554746479 @default.
• W2056523976 cites W1562998862 @default.
• W2056523976 cites W1568167591 @default.
• W2056523976 cites W1947487531 @default.
• W2056523976 cites W1964585383 @default.
• W2056523976 cites W1991262785 @default.
• W2056523976 cites W1993574643 @default.
• W2056523976 cites W2001692359 @default.
• W2056523976 cites W2008598406 @default.
• W2056523976 cites W2012569610 @default.
• W2056523976 cites W2013429292 @default.
• W2056523976 cites W2013738196 @default.
• W2056523976 cites W2018289835 @default.
• W2056523976 cites W2023930824 @default.
• W2056523976 cites W2025033304 @default.
• W2056523976 cites W2028429227 @default.
• W2056523976 cites W2035272773 @default.
• W2056523976 cites W2035394074 @default.
• W2056523976 cites W2036145275 @default.
• W2056523976 cites W2040358382 @default.
• W2056523976 cites W2047149593 @default.
• W2056523976 cites W2053633174 @default.
• W2056523976 cites W2060426330 @default.
• W2056523976 cites W2068771093 @default.
• W2056523976 cites W2068877244 @default.
• W2056523976 cites W2070322955 @default.
• W2056523976 cites W2071032007 @default.
• W2056523976 cites W2071931437 @default.
• W2056523976 cites W2078411762 @default.
• W2056523976 cites W2078512742 @default.
• W2056523976 cites W2087051342 @default.
• W2056523976 cites W2087298783 @default.
• W2056523976 cites W2088540736 @default.
• W2056523976 cites W2088833154 @default.
• W2056523976 cites W2093113620 @default.
• W2056523976 cites W2093273852 @default.
• W2056523976 cites W2093837825 @default.
• W2056523976 cites W2100837269 @default.
• W2056523976 cites W2100973053 @default.
• W2056523976 cites W2111691978 @default.
• W2056523976 cites W2153799259 @default.
• W2056523976 cites W2160061090 @default.
• W2056523976 cites W2220619187 @default.
• W2056523976 cites W2248158391 @default.
• W2056523976 cites W2276795069 @default.
• W2056523976 cites W2314282423 @default.
• W2056523976 cites W2338605836 @default.
• W2056523976 cites W2340411193 @default.
• W2056523976 cites W2411203475 @default.
• W2056523976 cites W2412430656 @default.
• W2056523976 cites W2419237941 @default.
• W2056523976 cites W97389648 @default.
• W2056523976 doi "https://doi.org/10.1172/jci113536" @default.
• W2056523976 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/442641" @default.
• W2056523976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2838525" @default.
• W2056523976 hasPublicationYear "1988" @default.
• W2056523976 type Work @default.
• W2056523976 sameAs 2056523976 @default.
• W2056523976 citedByCount "38" @default.
• W2056523976 countsByYear W20565239762012 @default.
• W2056523976 countsByYear W20565239762018 @default.
• W2056523976 crossrefType "journal-article" @default.
• W2056523976 hasAuthorship W2056523976A5004837178 @default.
• W2056523976 hasAuthorship W2056523976A5005094554 @default.
• W2056523976 hasAuthorship W2056523976A5040985491 @default.
• W2056523976 hasBestOaLocation W20565239761 @default.
• W2056523976 hasConcept C118716 @default.
• W2056523976 hasConcept C12927208 @default.
• W2056523976 hasConcept C153911025 @default.
• W2056523976 hasConcept C153939273 @default.
• W2056523976 hasConcept C170493617 @default.
• W2056523976 hasConcept C181199279 @default.
• W2056523976 hasConcept C185592680 @default.
• W2056523976 hasConcept C2777427919 @default.
• W2056523976 hasConcept C2778597717 @default.
• W2056523976 hasConcept C2779591629 @default.
• W2056523976 hasConcept C2780610907 @default.
• W2056523976 hasConcept C2781387506 @default.
• W2056523976 hasConcept C49773422 @default.
• W2056523976 hasConcept C55493867 @default.
• W2056523976 hasConcept C62478195 @default.
• W2056523976 hasConcept C80631254 @default.
• W2056523976 hasConcept C86803240 @default.
• W2056523976 hasConceptScore W2056523976C118716 @default.

• next