FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056526976> ?p ?o ?g. }

• W2056526976 endingPage "211" @default.
• W2056526976 startingPage "206" @default.
• W2056526976 abstract "□ This study describes the synthesis of a new generation of immunotoxins made by a noncovalent interaction between a monoclonal antibody derivatized with a dichlorotriazinic dye and the ribosomal inhibitor protein gelonin. The scheme of preparation has several advantages with respect to the traditional methods, which used heterobifunctional cross-linkers, such as a higher overall yield of production and the homogeneity of the obtained conjugate. Moreover, because no chemical derivatization of the gelonin was required, the unconjugated ribosome inactivating protein was recovered unaltered and therefore can be reused in other synthetic processes. This immunoconjugate was stable when tested in mouse serum and showed an interesting slow elimination rate when administered intravenously in mice. Although a high dye derivatization degree induced a modification of the specificity of the monoclonal antibody, the native specificity was restored after conjugation with gelonin. Furthermore the noncovalent linkage did not affect the gelonin inhibitory activity; in fact, the specific cytotoxic activity seemed to be similar to that of other disulfide-linked immunotoxins previously prepared in our laboratories. □ This study describes the synthesis of a new generation of immunotoxins made by a noncovalent interaction between a monoclonal antibody derivatized with a dichlorotriazinic dye and the ribosomal inhibitor protein gelonin. The scheme of preparation has several advantages with respect to the traditional methods, which used heterobifunctional cross-linkers, such as a higher overall yield of production and the homogeneity of the obtained conjugate. Moreover, because no chemical derivatization of the gelonin was required, the unconjugated ribosome inactivating protein was recovered unaltered and therefore can be reused in other synthetic processes. This immunoconjugate was stable when tested in mouse serum and showed an interesting slow elimination rate when administered intravenously in mice. Although a high dye derivatization degree induced a modification of the specificity of the monoclonal antibody, the native specificity was restored after conjugation with gelonin. Furthermore the noncovalent linkage did not affect the gelonin inhibitory activity; in fact, the specific cytotoxic activity seemed to be similar to that of other disulfide-linked immunotoxins previously prepared in our laboratories." @default.
• W2056526976 created "2016-06-24" @default.
• W2056526976 creator A5001339286 @default.
• W2056526976 creator A5003881266 @default.
• W2056526976 creator A5004472086 @default.
• W2056526976 creator A5004608542 @default.
• W2056526976 creator A5036607687 @default.
• W2056526976 creator A5059832414 @default.
• W2056526976 date "1994-02-01" @default.
• W2056526976 modified "2023-10-14" @default.
• W2056526976 title "A New Approach in the Synthesis of Immunotoxins: Ribosome Inactivating Protein Noncovalently Bound to Monoclonal Antibody" @default.
• W2056526976 cites W1490814033 @default.
• W2056526976 cites W1517902777 @default.
• W2056526976 cites W1773975057 @default.
• W2056526976 cites W1861217165 @default.
• W2056526976 cites W1936753325 @default.
• W2056526976 cites W1965172112 @default.
• W2056526976 cites W1965960922 @default.
• W2056526976 cites W1966515743 @default.
• W2056526976 cites W1978318684 @default.
• W2056526976 cites W1979858162 @default.
• W2056526976 cites W1992234269 @default.
• W2056526976 cites W1998827088 @default.
• W2056526976 cites W2011488579 @default.
• W2056526976 cites W2011651414 @default.
• W2056526976 cites W2017199460 @default.
• W2056526976 cites W2020880168 @default.
• W2056526976 cites W2031774008 @default.
• W2056526976 cites W2035099988 @default.
• W2056526976 cites W2038290059 @default.
• W2056526976 cites W2041998167 @default.
• W2056526976 cites W2045799781 @default.
• W2056526976 cites W2048464682 @default.
• W2056526976 cites W2053794928 @default.
• W2056526976 cites W2060281851 @default.
• W2056526976 cites W2080580789 @default.
• W2056526976 cites W2087281292 @default.
• W2056526976 cites W2089945610 @default.
• W2056526976 cites W2090272793 @default.
• W2056526976 cites W2100837269 @default.
• W2056526976 cites W4298235304 @default.
• W2056526976 doi "https://doi.org/10.1002/jps.2600830218" @default.
• W2056526976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8169790" @default.
• W2056526976 hasPublicationYear "1994" @default.
• W2056526976 type Work @default.
• W2056526976 sameAs 2056526976 @default.
• W2056526976 citedByCount "9" @default.
• W2056526976 crossrefType "journal-article" @default.
• W2056526976 hasAuthorship W2056526976A5001339286 @default.
• W2056526976 hasAuthorship W2056526976A5003881266 @default.
• W2056526976 hasAuthorship W2056526976A5004472086 @default.
• W2056526976 hasAuthorship W2056526976A5004608542 @default.
• W2056526976 hasAuthorship W2056526976A5036607687 @default.
• W2056526976 hasAuthorship W2056526976A5059832414 @default.
• W2056526976 hasConcept C104317684 @default.
• W2056526976 hasConcept C109316439 @default.
• W2056526976 hasConcept C115085202 @default.
• W2056526976 hasConcept C134306372 @default.
• W2056526976 hasConcept C153911025 @default.
• W2056526976 hasConcept C159654299 @default.
• W2056526976 hasConcept C179998833 @default.
• W2056526976 hasConcept C185592680 @default.
• W2056526976 hasConcept C197336794 @default.
• W2056526976 hasConcept C202751555 @default.
• W2056526976 hasConcept C203014093 @default.
• W2056526976 hasConcept C2776923573 @default.
• W2056526976 hasConcept C2778556491 @default.
• W2056526976 hasConcept C2780128948 @default.
• W2056526976 hasConcept C33923547 @default.
• W2056526976 hasConcept C43617362 @default.
• W2056526976 hasConcept C542903549 @default.
• W2056526976 hasConcept C55493867 @default.
• W2056526976 hasConcept C67705224 @default.
• W2056526976 hasConcept C86803240 @default.
• W2056526976 hasConcept C88478588 @default.
• W2056526976 hasConceptScore W2056526976C104317684 @default.
• W2056526976 hasConceptScore W2056526976C109316439 @default.
• W2056526976 hasConceptScore W2056526976C115085202 @default.
• W2056526976 hasConceptScore W2056526976C134306372 @default.
• W2056526976 hasConceptScore W2056526976C153911025 @default.
• W2056526976 hasConceptScore W2056526976C159654299 @default.
• W2056526976 hasConceptScore W2056526976C179998833 @default.
• W2056526976 hasConceptScore W2056526976C185592680 @default.
• W2056526976 hasConceptScore W2056526976C197336794 @default.
• W2056526976 hasConceptScore W2056526976C202751555 @default.
• W2056526976 hasConceptScore W2056526976C203014093 @default.
• W2056526976 hasConceptScore W2056526976C2776923573 @default.
• W2056526976 hasConceptScore W2056526976C2778556491 @default.
• W2056526976 hasConceptScore W2056526976C2780128948 @default.
• W2056526976 hasConceptScore W2056526976C33923547 @default.
• W2056526976 hasConceptScore W2056526976C43617362 @default.
• W2056526976 hasConceptScore W2056526976C542903549 @default.
• W2056526976 hasConceptScore W2056526976C55493867 @default.
• W2056526976 hasConceptScore W2056526976C67705224 @default.
• W2056526976 hasConceptScore W2056526976C86803240 @default.
• W2056526976 hasConceptScore W2056526976C88478588 @default.
• W2056526976 hasIssue "2" @default.
• W2056526976 hasLocation W20565269761 @default.

• next