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- W2056528983 abstract "As research on the biodegradation of benzo[a]pyrene (B[a]P) shifts from laboratory to field based studies, a need has arisen for reproducible, economical, and accurate methods for extraction and quantification of B[a]P. This study describes a method for the analysis of B[a]P using batch extraction techniques with quantification by reversed phase high performance thin layer chromatography (HPTLC) and densitometry. A loam soil was fortified with B[a]P at a level of 100 mg B[a]P kg−1 soil. Several solvents were evaluated as potential soil extraction systems including: acetone, acetonitrile, ethyl acetate, methanol, and methylene chloride:acetone (1:1). Reverse phase thin layer chromatography of B[a]P was performed on hydrocarbon impregnated, C18 reverse phase plates using methanol:acetonitrile (1:1) as the developing solvent. The Rf for B[a]P in this system was found to be 0.52. Recovery of B[a]P from soil ranged from 11% with methanol to 84% with ethyl acetate." @default.
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- W2056528983 date "2000-01-01" @default.
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- W2056528983 title "EXTRACTION AND QUANTIFICATION OF BENZO[a]PYRENE IN SOIL BY REVERSED PHASE THIN LAYER CHROMATOGRAPHY" @default.
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- W2056528983 doi "https://doi.org/10.1081/jlc-100101474" @default.
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