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• W2056537467 abstract "In this study, we investigated the effect of Panax ginseng root aqueous extracts upon inducible nitric oxide synthesis in RAW 264.7 cells. Panax ginseng root extract has been used in the Asian world for centuries as a traditional herb to enhance physical strength and resistance and is becoming more and more popular in Europe and North America. Incubation of murine macrophages (RAW 264.7 cells) with increasing amounts of aqueous extracts of Panax ginseng (0.05 – 0.8 μg μl−1) showed a dose dependent stimulation of inducible nitric oxide synthesis. Polysaccharides isolated from Panax ginseng showed strong stimulation of inducible nitric oxide synthesis, whereas a triterpene-enriched fraction from an aqueous extract of Panax ginseng did not show any stimulation. Inducible nitric oxide synthase protein expression was enhanced in a dose dependent manner as revealed by immunoblotting when cells were incubated with increasing amounts of Panax ginseng extract. This was associated with an incline in inducible nitric oxide synthase mRNA-levels as determined by semiquantitative polymerase chain reaction and electromobility shift assay studies indicated enhanced nuclear factor-κB DNA binding activity. As nitric oxide plays an important role in immune function, Panax ginseng treatment could modulate several aspects of host defense mechanisms due to stimulation of the inducible nitric oxide synthase. British Journal of Pharmacology (2001) 134, 1663–1670; doi:10.1038/sj.bjp.0704425" @default.
• W2056537467 created "2016-06-24" @default.
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• W2056537467 date "2001-12-01" @default.
• W2056537467 modified "2023-09-26" @default.
• W2056537467 title "Stimulation of nitric oxide synthesis by the aqueous extract of<i>Panax ginseng</i>root in RAW 264.7 cells" @default.
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• W2056537467 doi "https://doi.org/10.1038/sj.bjp.0704425" @default.
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