Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056545436> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2056545436 endingPage "256" @default.
- W2056545436 startingPage "243" @default.
- W2056545436 abstract "Binding of troponin to Cibacron Blue F3GA-agarose column and its selective release from the gel in the presence of 0.5 M KCl provides the basis for a new purification method. The two-step procedure consists of isoelectric precipitation of tropomyosin and chromatography of the resultant crude troponin supernatant on Affi-Gel Blue column. Adsorption of troponin to the immobilized dye appears to occur through the troponin-T subunit. Troponin-I and troponin-C do not bind to the blue agarose column, whereas troponin-T binds to it very tightly. Binding of the dye to troponin-T prevents formation of troponin-T-troponin-C complex, but does not interfere with direct interaction of troponin-T with troponin-I. The activity of troponin in conferring calcium sensitivity on actomyosin ATPase is not affected by Cibacron Blue. Circular dichroism and difference absorption measurements of complexes of the blue dye with troponin and its subunits reveal the presence of a tight binding site on whole troponin and on troponin-T (KA greater than or equal to 10(6) M). The existence of weak binding sites for the dye on troponin and all of its subunits is deduced from difference absorption studies. Cibacron Blue appears to be a sensitive probe for subunit interactions in troponin." @default.
- W2056545436 created "2016-06-24" @default.
- W2056545436 creator A5031937602 @default.
- W2056545436 creator A5038346978 @default.
- W2056545436 creator A5038398169 @default.
- W2056545436 creator A5062138355 @default.
- W2056545436 date "1980-06-01" @default.
- W2056545436 modified "2023-09-27" @default.
- W2056545436 title "The interaction of cibacron blue F3GA with troponin and its subunits" @default.
- W2056545436 cites W141855200 @default.
- W2056545436 cites W1488584170 @default.
- W2056545436 cites W1538692246 @default.
- W2056545436 cites W1556587164 @default.
- W2056545436 cites W1598846697 @default.
- W2056545436 cites W1625660792 @default.
- W2056545436 cites W1900935399 @default.
- W2056545436 cites W1988012975 @default.
- W2056545436 cites W1993662939 @default.
- W2056545436 cites W2000411386 @default.
- W2056545436 cites W2003186676 @default.
- W2056545436 cites W2007615331 @default.
- W2056545436 cites W2020284683 @default.
- W2056545436 cites W2022833698 @default.
- W2056545436 cites W2041623457 @default.
- W2056545436 cites W2044873655 @default.
- W2056545436 cites W2054774765 @default.
- W2056545436 cites W2058321065 @default.
- W2056545436 cites W2058835652 @default.
- W2056545436 cites W2067881860 @default.
- W2056545436 cites W2071196829 @default.
- W2056545436 cites W2153435210 @default.
- W2056545436 cites W2331838300 @default.
- W2056545436 doi "https://doi.org/10.1016/0005-2795(80)90253-6" @default.
- W2056545436 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6893162" @default.
- W2056545436 hasPublicationYear "1980" @default.
- W2056545436 type Work @default.
- W2056545436 sameAs 2056545436 @default.
- W2056545436 citedByCount "17" @default.
- W2056545436 crossrefType "journal-article" @default.
- W2056545436 hasAuthorship W2056545436A5031937602 @default.
- W2056545436 hasAuthorship W2056545436A5038346978 @default.
- W2056545436 hasAuthorship W2056545436A5038398169 @default.
- W2056545436 hasAuthorship W2056545436A5062138355 @default.
- W2056545436 hasConcept C115725540 @default.
- W2056545436 hasConcept C133927893 @default.
- W2056545436 hasConcept C144469398 @default.
- W2056545436 hasConcept C164705383 @default.
- W2056545436 hasConcept C179247698 @default.
- W2056545436 hasConcept C181199279 @default.
- W2056545436 hasConcept C185592680 @default.
- W2056545436 hasConcept C2778476535 @default.
- W2056545436 hasConcept C36036425 @default.
- W2056545436 hasConcept C43617362 @default.
- W2056545436 hasConcept C500558357 @default.
- W2056545436 hasConcept C50476208 @default.
- W2056545436 hasConcept C55493867 @default.
- W2056545436 hasConcept C71924100 @default.
- W2056545436 hasConcept C8880881 @default.
- W2056545436 hasConceptScore W2056545436C115725540 @default.
- W2056545436 hasConceptScore W2056545436C133927893 @default.
- W2056545436 hasConceptScore W2056545436C144469398 @default.
- W2056545436 hasConceptScore W2056545436C164705383 @default.
- W2056545436 hasConceptScore W2056545436C179247698 @default.
- W2056545436 hasConceptScore W2056545436C181199279 @default.
- W2056545436 hasConceptScore W2056545436C185592680 @default.
- W2056545436 hasConceptScore W2056545436C2778476535 @default.
- W2056545436 hasConceptScore W2056545436C36036425 @default.
- W2056545436 hasConceptScore W2056545436C43617362 @default.
- W2056545436 hasConceptScore W2056545436C500558357 @default.
- W2056545436 hasConceptScore W2056545436C50476208 @default.
- W2056545436 hasConceptScore W2056545436C55493867 @default.
- W2056545436 hasConceptScore W2056545436C71924100 @default.
- W2056545436 hasConceptScore W2056545436C8880881 @default.
- W2056545436 hasIssue "2" @default.
- W2056545436 hasLocation W20565454361 @default.
- W2056545436 hasLocation W20565454362 @default.
- W2056545436 hasOpenAccess W2056545436 @default.
- W2056545436 hasPrimaryLocation W20565454361 @default.
- W2056545436 hasRelatedWork W1986570242 @default.
- W2056545436 hasRelatedWork W2013195452 @default.
- W2056545436 hasRelatedWork W2045229214 @default.
- W2056545436 hasRelatedWork W2046543266 @default.
- W2056545436 hasRelatedWork W2049996685 @default.
- W2056545436 hasRelatedWork W2056545436 @default.
- W2056545436 hasRelatedWork W2082155590 @default.
- W2056545436 hasRelatedWork W2334709023 @default.
- W2056545436 hasRelatedWork W3004814566 @default.
- W2056545436 hasRelatedWork W2174574175 @default.
- W2056545436 hasVolume "623" @default.
- W2056545436 isParatext "false" @default.
- W2056545436 isRetracted "false" @default.
- W2056545436 magId "2056545436" @default.
- W2056545436 workType "article" @default.