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- W2056547706 abstract "Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpes-virus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma cells with splenocytes of BALB/c mice previously immunized with purified BHV-4. A modified indirect fluorescent antibody test (IFAT) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. The selected Mabs were tested by the same IFAT against a panel of BHV-4 field isolates and against bovine herpesvirus-1, bovine herpesvirus-2 and alcelaphine herpesvirus-1 (AHV-1). Comparison of BHV-4 field isolates with Mabs confirmed their close antigenic relationships, but slight antigenic differences were observed between different isolates. One of the Mabs also reacted against AHV-1, indicating an antigenic relationship between BHV-4 and AHV-1. None of the Mabs reacting with BHV-4 possessed neutralizing activity against the strain used for immunization." @default.
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- W2056547706 date "1989-04-01" @default.
- W2056547706 modified "2023-10-02" @default.
- W2056547706 title "Production and characterization of monoclonal antibodies to bovid herpesvirus-4" @default.
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- W2056547706 doi "https://doi.org/10.1016/0378-1135(89)90096-5" @default.
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