Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056548670> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2056548670 endingPage "159" @default.
- W2056548670 startingPage "149" @default.
- W2056548670 abstract "Rapid clonal growth of primary cultures of human costal chondrocytes in a defined medium has been achieved. The basal nutrient medium used for such growth is MCDB 104. It is prepared without linoleic acid and supplemented with 1 microgram/ml insulin, 100 ng/ml fibroblast growth factor, 1.0 x 10(-7) M dexamethasone, and 5 micrograms/ml mixed lipids, presented to the cells in the form of liposomes. The lipid supplement contains soybean lecithin, cholesterol, sphingomyelin, vitamin E, and vitamin E acetate. No expression of cartilage-like differentiation occurs in the defined medium. However, colonies grown for several days in the defined medium and then grown for an additional period of time in medium F12 supplemented with fetal bovine serum and chicken embryo extract synthesize large amounts of refractile matrix that is stained intensely by acidified alcian green, thus demonstrating that the cells growing in the defined medium are capable of expressing cartilage matrix in a permissive environment. Good clonal growth and expression of differentiation can also be obtained by inoculating primary cultures of human chondrocytes directly into the F12-serum-embryo extract medium." @default.
- W2056548670 created "2016-06-24" @default.
- W2056548670 creator A5045454180 @default.
- W2056548670 creator A5051427909 @default.
- W2056548670 date "1983-02-01" @default.
- W2056548670 modified "2023-09-23" @default.
- W2056548670 title "Clonal growth of primary cultures of human hyaline chondrocytes in a defined medium" @default.
- W2056548670 cites W1974396346 @default.
- W2056548670 cites W1981111084 @default.
- W2056548670 cites W1983167228 @default.
- W2056548670 cites W1988922507 @default.
- W2056548670 cites W2012118931 @default.
- W2056548670 cites W2012738478 @default.
- W2056548670 cites W2013655175 @default.
- W2056548670 cites W2018437033 @default.
- W2056548670 cites W2028662074 @default.
- W2056548670 cites W2036305879 @default.
- W2056548670 cites W2036513761 @default.
- W2056548670 cites W2064514209 @default.
- W2056548670 cites W2098763078 @default.
- W2056548670 cites W2110032242 @default.
- W2056548670 cites W2151370338 @default.
- W2056548670 cites W2153929601 @default.
- W2056548670 cites W2155059288 @default.
- W2056548670 cites W2233425570 @default.
- W2056548670 cites W2272947817 @default.
- W2056548670 cites W936306301 @default.
- W2056548670 doi "https://doi.org/10.1016/0309-1651(83)90028-0" @default.
- W2056548670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6220818" @default.
- W2056548670 hasPublicationYear "1983" @default.
- W2056548670 type Work @default.
- W2056548670 sameAs 2056548670 @default.
- W2056548670 citedByCount "25" @default.
- W2056548670 countsByYear W20565486702012 @default.
- W2056548670 countsByYear W20565486702020 @default.
- W2056548670 crossrefType "journal-article" @default.
- W2056548670 hasAuthorship W2056548670A5045454180 @default.
- W2056548670 hasAuthorship W2056548670A5051427909 @default.
- W2056548670 hasConcept C105702510 @default.
- W2056548670 hasConcept C106487976 @default.
- W2056548670 hasConcept C185592680 @default.
- W2056548670 hasConcept C196843134 @default.
- W2056548670 hasConcept C202751555 @default.
- W2056548670 hasConcept C2780550940 @default.
- W2056548670 hasConcept C2780919596 @default.
- W2056548670 hasConcept C43617362 @default.
- W2056548670 hasConcept C523546767 @default.
- W2056548670 hasConcept C54355233 @default.
- W2056548670 hasConcept C55493867 @default.
- W2056548670 hasConcept C81885089 @default.
- W2056548670 hasConcept C86803240 @default.
- W2056548670 hasConcept C95444343 @default.
- W2056548670 hasConcept C98648569 @default.
- W2056548670 hasConceptScore W2056548670C105702510 @default.
- W2056548670 hasConceptScore W2056548670C106487976 @default.
- W2056548670 hasConceptScore W2056548670C185592680 @default.
- W2056548670 hasConceptScore W2056548670C196843134 @default.
- W2056548670 hasConceptScore W2056548670C202751555 @default.
- W2056548670 hasConceptScore W2056548670C2780550940 @default.
- W2056548670 hasConceptScore W2056548670C2780919596 @default.
- W2056548670 hasConceptScore W2056548670C43617362 @default.
- W2056548670 hasConceptScore W2056548670C523546767 @default.
- W2056548670 hasConceptScore W2056548670C54355233 @default.
- W2056548670 hasConceptScore W2056548670C55493867 @default.
- W2056548670 hasConceptScore W2056548670C81885089 @default.
- W2056548670 hasConceptScore W2056548670C86803240 @default.
- W2056548670 hasConceptScore W2056548670C95444343 @default.
- W2056548670 hasConceptScore W2056548670C98648569 @default.
- W2056548670 hasIssue "2" @default.
- W2056548670 hasLocation W20565486701 @default.
- W2056548670 hasLocation W20565486702 @default.
- W2056548670 hasOpenAccess W2056548670 @default.
- W2056548670 hasPrimaryLocation W20565486701 @default.
- W2056548670 hasRelatedWork W2012346633 @default.
- W2056548670 hasRelatedWork W2012347861 @default.
- W2056548670 hasRelatedWork W2015183309 @default.
- W2056548670 hasRelatedWork W2063285791 @default.
- W2056548670 hasRelatedWork W2072703099 @default.
- W2056548670 hasRelatedWork W2079407183 @default.
- W2056548670 hasRelatedWork W2397734196 @default.
- W2056548670 hasRelatedWork W2411165638 @default.
- W2056548670 hasRelatedWork W2419474872 @default.
- W2056548670 hasRelatedWork W4285813724 @default.
- W2056548670 hasVolume "7" @default.
- W2056548670 isParatext "false" @default.
- W2056548670 isRetracted "false" @default.
- W2056548670 magId "2056548670" @default.
- W2056548670 workType "article" @default.