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• W2056549843 abstract "Addition of thyrotropin-releasing hormone (TRH) (10 nM to 10 microM) to bovine anterior pituitary cells labelled with [3H]inositol decreased the radioactivity in inositol-containing lipids and increased it in inositol phosphates. TRH also increased the cytoplasmic calcium concentration biphasically. At TRH concentrations below 10 nM, the increase was sustained and sensitive to inhibitors of calcium influx through voltage-gated channels, whereas concentrations over 10 nM elicited in addition a rapid transient increase in calcium, which was relatively insensitive to such inhibition. Incubation of the cells in medium containing 25 mM KCl increased the cytoplasmic calcium concentration by stimulating influx through voltage-gated channels, and markedly enhanced the initial transient increase of calcium seen at TRH concentrations above 10 nM. It did not affect the generation of InsP3 and it also enhanced the calcium response to ionomycin. It is suggested that stimulation of calcium entry through voltage-gated channels can increase the amount of calcium available for mobilisation by TRH." @default.
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• W2056549843 date "1989-09-01" @default.
• W2056549843 modified "2023-09-27" @default.
• W2056549843 title "The effects of thyrotropin-releasing hormone and potassium depolarization on phosphoinositide metabolism and cytoplasmic calcium in bovine pituitary cells" @default.
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• W2056549843 doi "https://doi.org/10.1016/0167-4889(89)90134-1" @default.
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