Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056551350> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2056551350 endingPage "699" @default.
- W2056551350 startingPage "692" @default.
- W2056551350 abstract "Delivery of genes into hematopoietic progenitor cells offers an attractive means for the introduction of corrective or protective genes into cells of both the myeloid and lymphoid lineage. Previously, investigators have often used murine retroviral vectors for gene delivery which require cells to be cycling for efficient delivery. We describe a nonviral method of gene delivery using particle-mediated gene transfer to obviate many disadvantages of viral vectors related to safety, production costs and the need for cell cycle proliferation. Using a CMV-CAT reporter plasmid, we show transfection of highly purified CD34+ cells isolated from umbilical cord blood. Effective gene transfer was shown in unstimulated and in growth-stimulated cells. Fol- lowing transfection with a neomycin resistance gene, differentiation into cells of the myeloid lineage was observed, assayed by CFU-GM in the presence of G-418. Both unstimulated and stimulated cells gave rise to CFU-GM in the presence of G-418, indicating that stable expression of the neomycin resistance gene was maintained in early progenitors. These results demonstrate that particle-mediated gene transfer into human hematopoietic cells from umbilical cord blood can be achieved without affecting their CFU-GM differentiation potential. This gene transfer method offers an alternative approach to gene therapy studies involving human hematopoietic progenitor cells." @default.
- W2056551350 created "2016-06-24" @default.
- W2056551350 creator A5012347503 @default.
- W2056551350 creator A5015205644 @default.
- W2056551350 creator A5020997464 @default.
- W2056551350 creator A5036055474 @default.
- W2056551350 creator A5049128212 @default.
- W2056551350 creator A5050109992 @default.
- W2056551350 creator A5052044401 @default.
- W2056551350 creator A5052126493 @default.
- W2056551350 creator A5061211279 @default.
- W2056551350 date "1998-05-01" @default.
- W2056551350 modified "2023-10-16" @default.
- W2056551350 title "Gene transfer into human umbilical cord blood-derived CD34+ cells by particle-mediated gene transfer" @default.
- W2056551350 doi "https://doi.org/10.1038/sj.gt.3300656" @default.
- W2056551350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9797875" @default.
- W2056551350 hasPublicationYear "1998" @default.
- W2056551350 type Work @default.
- W2056551350 sameAs 2056551350 @default.
- W2056551350 citedByCount "12" @default.
- W2056551350 countsByYear W20565513502012 @default.
- W2056551350 countsByYear W20565513502014 @default.
- W2056551350 countsByYear W20565513502018 @default.
- W2056551350 countsByYear W20565513502022 @default.
- W2056551350 countsByYear W20565513502023 @default.
- W2056551350 crossrefType "journal-article" @default.
- W2056551350 hasAuthorship W2056551350A5012347503 @default.
- W2056551350 hasAuthorship W2056551350A5015205644 @default.
- W2056551350 hasAuthorship W2056551350A5020997464 @default.
- W2056551350 hasAuthorship W2056551350A5036055474 @default.
- W2056551350 hasAuthorship W2056551350A5049128212 @default.
- W2056551350 hasAuthorship W2056551350A5050109992 @default.
- W2056551350 hasAuthorship W2056551350A5052044401 @default.
- W2056551350 hasAuthorship W2056551350A5052126493 @default.
- W2056551350 hasAuthorship W2056551350A5061211279 @default.
- W2056551350 hasBestOaLocation W20565513501 @default.
- W2056551350 hasConcept C10205521 @default.
- W2056551350 hasConcept C104317684 @default.
- W2056551350 hasConcept C109159458 @default.
- W2056551350 hasConcept C111599444 @default.
- W2056551350 hasConcept C135983454 @default.
- W2056551350 hasConcept C150194340 @default.
- W2056551350 hasConcept C153911025 @default.
- W2056551350 hasConcept C161733203 @default.
- W2056551350 hasConcept C201750760 @default.
- W2056551350 hasConcept C203014093 @default.
- W2056551350 hasConcept C2776955114 @default.
- W2056551350 hasConcept C2779282312 @default.
- W2056551350 hasConcept C2780914630 @default.
- W2056551350 hasConcept C28328180 @default.
- W2056551350 hasConcept C32470452 @default.
- W2056551350 hasConcept C40767141 @default.
- W2056551350 hasConcept C54009773 @default.
- W2056551350 hasConcept C54355233 @default.
- W2056551350 hasConcept C86803240 @default.
- W2056551350 hasConcept C95444343 @default.
- W2056551350 hasConceptScore W2056551350C10205521 @default.
- W2056551350 hasConceptScore W2056551350C104317684 @default.
- W2056551350 hasConceptScore W2056551350C109159458 @default.
- W2056551350 hasConceptScore W2056551350C111599444 @default.
- W2056551350 hasConceptScore W2056551350C135983454 @default.
- W2056551350 hasConceptScore W2056551350C150194340 @default.
- W2056551350 hasConceptScore W2056551350C153911025 @default.
- W2056551350 hasConceptScore W2056551350C161733203 @default.
- W2056551350 hasConceptScore W2056551350C201750760 @default.
- W2056551350 hasConceptScore W2056551350C203014093 @default.
- W2056551350 hasConceptScore W2056551350C2776955114 @default.
- W2056551350 hasConceptScore W2056551350C2779282312 @default.
- W2056551350 hasConceptScore W2056551350C2780914630 @default.
- W2056551350 hasConceptScore W2056551350C28328180 @default.
- W2056551350 hasConceptScore W2056551350C32470452 @default.
- W2056551350 hasConceptScore W2056551350C40767141 @default.
- W2056551350 hasConceptScore W2056551350C54009773 @default.
- W2056551350 hasConceptScore W2056551350C54355233 @default.
- W2056551350 hasConceptScore W2056551350C86803240 @default.
- W2056551350 hasConceptScore W2056551350C95444343 @default.
- W2056551350 hasIssue "5" @default.
- W2056551350 hasLocation W20565513501 @default.
- W2056551350 hasLocation W20565513502 @default.
- W2056551350 hasOpenAccess W2056551350 @default.
- W2056551350 hasPrimaryLocation W20565513501 @default.
- W2056551350 hasRelatedWork W2010743927 @default.
- W2056551350 hasRelatedWork W2053332535 @default.
- W2056551350 hasRelatedWork W2055138950 @default.
- W2056551350 hasRelatedWork W2080280565 @default.
- W2056551350 hasRelatedWork W2150071496 @default.
- W2056551350 hasRelatedWork W2313748271 @default.
- W2056551350 hasRelatedWork W3037848594 @default.
- W2056551350 hasRelatedWork W4232065991 @default.
- W2056551350 hasRelatedWork W4240611894 @default.
- W2056551350 hasRelatedWork W985386580 @default.
- W2056551350 hasVolume "5" @default.
- W2056551350 isParatext "false" @default.
- W2056551350 isRetracted "false" @default.
- W2056551350 magId "2056551350" @default.
- W2056551350 workType "article" @default.