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• W2056552225 abstract "The identification of T cell epitopes is crucial for the understanding of the host response during infections with pathogenic microorganisms. Generally, the identification of relevant T cell responses is based on the analysis of T cell lines propagated in vitro. We used an ex vivo approach for the analysis of the CD8 T cell response against Listeria monocytogenes that is based upon the fractionation of naturally processed antigenic peptides and subsequent analysis with T cells in an enzyme-linked immunospot (ELISPOT) assay. Our data indicate that the direct ex vivo ELISPOT analysis of peptides extracted from infected tissues represents a versatile and potent test system for the analysis of the CD8 T cell immunome of microorganisms that furthermore requires neither the knowledge of the microbial genome nor of the specificity of responding T cells." @default.
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• W2056552225 date "2003-04-01" @default.
• W2056552225 modified "2023-09-26" @default.
• W2056552225 title "CD8 T cell immunome analysis of<i>Listeria monocytogenes</i>" @default.
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• W2056552225 doi "https://doi.org/10.1016/s0928-8244(02)00450-9" @default.
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