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• W2056553858 endingPage "331" @default.
• W2056553858 startingPage "331" @default.
• W2056553858 abstract "<h3>Objective:</h3> To assay drugs that modify pigmentation (ie, agents that either enhance or decrease pigmentation) in a predictive in vitro model, as such drugs have been previously studied in vivo on human and animal skin. <h3>Design:</h3> An epidermis was reconstructed with human keratinocytes and melanocytes. Epidermal reconstructs with melanocytes were made according to a variant of the model of Pruniéras and colleagues. Reagents that alter melanogenesis such as isobutyl methylxanthine, 1-oleoyl-2-acetyl-sn-glycerol, kojic acid, and mequinol were introduced in the culture medium or used topically after 8 days of culture at the air-liquid interface. <h3>Main Outcome Measures:</h3> Macroscopic, colorimetric, histological (Fontana-Masson stain and dopa reaction), and biochemical studies were performed. <h3>Results:</h3> The effect of pigment modifiers was demonstrated ex vivo as expected from in vivo data on skin color, numbers of melanocytes, melanosome transfer, and melanin content. <h3>Conclusion:</h3> These results will permit other investigators to use this model to assess the efficacy of novel molecules that alter pigmentation. Arch Dermatol. 1997;133:331-336" @default.
• W2056553858 created "2016-06-24" @default.
• W2056553858 creator A5001501621 @default.
• W2056553858 date "1997-03-01" @default.
• W2056553858 modified "2023-09-25" @default.
• W2056553858 title "Use of Human Skin Reconstructs in the Study of Pigment Modifiers" @default.
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• W2056553858 doi "https://doi.org/10.1001/archderm.1997.03890390069009" @default.
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