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• W2056556061 abstract "A spectrophotometric method is described which when used as directed provides routine precision of about ±0.5% in degree of gas binding and about ±0.1% in partial pressure of the gas. The method is an elaboration of methods now in use, but has been improved both by the incorporation of a partially bored stopcock for introduction of the gas and by elaboration of manipulative procedures to reduce protein loss either via denaturation or through the production of oxidized forms. Manipulative errors have been reduced to the point that the limiting error is that of a high-grade spectrophotometer. The method is convenient and is not time consuming. Further improvements in spectrometric methods may be possible, but only with very elaborate attention to manipulative errors and only with the use of devices of higher photometric precision than most conventional commercial spectrophotometers. The method makes it now possible to determine directly with precision isotherms for the binding of carbon monoxide to myoglobin." @default.
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• W2056556061 date "1967-01-01" @default.
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• W2056556061 title "Equilibrium measurement in the reactions of heme-proteins with gaseous ligands" @default.
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• W2056556061 doi "https://doi.org/10.1016/0003-2697(67)90065-6" @default.
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