Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056556674> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2056556674 endingPage "109" @default.
- W2056556674 startingPage "101" @default.
- W2056556674 abstract "Helix aspersa neuronal cell membranes contain distinct serotonin (5-HT) and dopamine (DA) sensitive adenylate cyclases. We have taken advantage of the fact that in this system, both in vitro (enzymatic assays) and in vivo (electrophysiological measurements) experiments can be used to explore the GTP dependence and the pharmacological properties of this neurotransmitter-sensitive enzyme system. The first property was studied using non-hydrolysable GTP analogs (guanosine 5′-O-(3-thio-triphosphate) or GTPγS, and guanosine 5′-imido diphosphate or Gpp(NH)p). In vitro, these two components stimulate the enzyme activity but with different potencies (Kapparent = 10−8 to 5 × 10−8 M for GTPγS, and 10−5 M for Gpp(NH)p). Intracellular injections of GTPγS, but not of Gpp(NH)p, produced an electrophysiological response similar to the one elicited by 5-HT and DA. These results imply that, even in the presence of the high endogenous GTP concentration normally present in the cell (10−3 M), GTPγS may bind to the GTP-binding protein. Such an interpretation is consistent with the in vitro competition experiments between GTP and GTPγS for adenylate cyclase activation. The pharmacology of 5-HT and DA receptors involved in adenylate cyclase stimulation and electrophysiological responses was studied. Serotoninergic antagonists and neuroleptics inhibited the 5-HT-sensitive adenylate cyclase in a stereospecific manner. However, their inhibition was not simply competitive. Our results suggest that they irreversibly bind a component localized on the cytoplasmic side of the membrane. Unexpectedly, the DA receptor coupled with adenylate cyclase was insensitive to any of the several antagonists tested." @default.
- W2056556674 created "2016-06-24" @default.
- W2056556674 creator A5013649152 @default.
- W2056556674 creator A5034172104 @default.
- W2056556674 creator A5045863654 @default.
- W2056556674 date "1986-11-01" @default.
- W2056556674 modified "2023-10-03" @default.
- W2056556674 title "Serotonin- and dopamine-sensitive adenylate cyclase in molluscan nervous system. Biochemical and electrophysiological analysis of the pharmacological properties and the GTP-dependence" @default.
- W2056556674 cites W1487187369 @default.
- W2056556674 cites W1508673049 @default.
- W2056556674 cites W1533636181 @default.
- W2056556674 cites W1984012040 @default.
- W2056556674 cites W1987193373 @default.
- W2056556674 cites W2001233326 @default.
- W2056556674 cites W2001812683 @default.
- W2056556674 cites W2007669353 @default.
- W2056556674 cites W2012294164 @default.
- W2056556674 cites W2012609110 @default.
- W2056556674 cites W2023916700 @default.
- W2056556674 cites W2030905853 @default.
- W2056556674 cites W2046879236 @default.
- W2056556674 cites W2060172635 @default.
- W2056556674 cites W2068877244 @default.
- W2056556674 cites W2074412543 @default.
- W2056556674 cites W2159244857 @default.
- W2056556674 cites W2160732186 @default.
- W2056556674 cites W2316148297 @default.
- W2056556674 doi "https://doi.org/10.1016/0169-328x(86)90001-x" @default.
- W2056556674 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3791004" @default.
- W2056556674 hasPublicationYear "1986" @default.
- W2056556674 type Work @default.
- W2056556674 sameAs 2056556674 @default.
- W2056556674 citedByCount "16" @default.
- W2056556674 crossrefType "journal-article" @default.
- W2056556674 hasAuthorship W2056556674A5013649152 @default.
- W2056556674 hasAuthorship W2056556674A5034172104 @default.
- W2056556674 hasAuthorship W2056556674A5045863654 @default.
- W2056556674 hasConcept C118716 @default.
- W2056556674 hasConcept C12554922 @default.
- W2056556674 hasConcept C134018914 @default.
- W2056556674 hasConcept C169760540 @default.
- W2056556674 hasConcept C170493617 @default.
- W2056556674 hasConcept C180526460 @default.
- W2056556674 hasConcept C181199279 @default.
- W2056556674 hasConcept C185263204 @default.
- W2056556674 hasConcept C2775864247 @default.
- W2056556674 hasConcept C2776638253 @default.
- W2056556674 hasConcept C2777995097 @default.
- W2056556674 hasConcept C2780069284 @default.
- W2056556674 hasConcept C37000724 @default.
- W2056556674 hasConcept C513476851 @default.
- W2056556674 hasConcept C55493867 @default.
- W2056556674 hasConcept C86803240 @default.
- W2056556674 hasConceptScore W2056556674C118716 @default.
- W2056556674 hasConceptScore W2056556674C12554922 @default.
- W2056556674 hasConceptScore W2056556674C134018914 @default.
- W2056556674 hasConceptScore W2056556674C169760540 @default.
- W2056556674 hasConceptScore W2056556674C170493617 @default.
- W2056556674 hasConceptScore W2056556674C180526460 @default.
- W2056556674 hasConceptScore W2056556674C181199279 @default.
- W2056556674 hasConceptScore W2056556674C185263204 @default.
- W2056556674 hasConceptScore W2056556674C2775864247 @default.
- W2056556674 hasConceptScore W2056556674C2776638253 @default.
- W2056556674 hasConceptScore W2056556674C2777995097 @default.
- W2056556674 hasConceptScore W2056556674C2780069284 @default.
- W2056556674 hasConceptScore W2056556674C37000724 @default.
- W2056556674 hasConceptScore W2056556674C513476851 @default.
- W2056556674 hasConceptScore W2056556674C55493867 @default.
- W2056556674 hasConceptScore W2056556674C86803240 @default.
- W2056556674 hasIssue "2" @default.
- W2056556674 hasLocation W20565566741 @default.
- W2056556674 hasLocation W20565566742 @default.
- W2056556674 hasOpenAccess W2056556674 @default.
- W2056556674 hasPrimaryLocation W20565566741 @default.
- W2056556674 hasRelatedWork W148461917 @default.
- W2056556674 hasRelatedWork W1510838637 @default.
- W2056556674 hasRelatedWork W2010504109 @default.
- W2056556674 hasRelatedWork W2032827312 @default.
- W2056556674 hasRelatedWork W2038792198 @default.
- W2056556674 hasRelatedWork W2048535559 @default.
- W2056556674 hasRelatedWork W2050202579 @default.
- W2056556674 hasRelatedWork W2055683147 @default.
- W2056556674 hasRelatedWork W2170189971 @default.
- W2056556674 hasRelatedWork W2194300488 @default.
- W2056556674 hasVolume "1" @default.
- W2056556674 isParatext "false" @default.
- W2056556674 isRetracted "false" @default.
- W2056556674 magId "2056556674" @default.
- W2056556674 workType "article" @default.