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• W2056556701 abstract "1. The free-flow concentration profile of Gln along the proximal convolution was determined by micropuncture. SNGFR was measured by [3H]inulin, glutamine by ultramicro column chromatography. Reabsorption kinetics were determined by microperfusion of the proximal convolution. The equilibrium perfusion fluids contained [3H]inulin and l-[14C]Gln. 2. Gln-reabsorption is saturable. Kinetic constants are: Jmax = 1.55 (±0.1 SEM) nmol-m−1-sec−1; Km = 2.1 (±0.13 SEM) mmol·1−1. 3. Free-flow concentration profiles predicted from these constants agree with those measured experimentally to a great extent. The endproximal steady state concentration amounts to about 20 μmol.1−1 at normal Gln plasma concentrations of 600 μmol.1−1. The endproximal fractional reabsorption amounts to more than 96°% up to 6 mmol.1− Gln plasma concentration. 4. Gln resorption from microperfusate is neither influenced by 2-oxo-glutarate, by mercaptopicolinic acid nor by acute respiratory acidosis. 5. It is concluded that Gln is reabsorbed from the proximal convolution nearly completely at normal as well as at up to 10-fold elevated Gln plasma concentrations. Acute respiratory acidosis and inhibition of cellular gluconeogenesis and mitochondrial Gln uptake does not seem to influence apparent resorption kinetics of Gln." @default.
• W2056556701 created "2016-06-24" @default.
• W2056556701 creator A5072537786 @default.
• W2056556701 date "1980-01-01" @default.
• W2056556701 modified "2023-10-15" @default.
• W2056556701 title "Tubular reabsorption of l-glutamine studied by free-flow micropuncture and microperfusion of rat kidney" @default.
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• W2056556701 doi "https://doi.org/10.1016/0020-711x(80)90034-8" @default.
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