FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056556864> ?p ?o ?g. }

• W2056556864 endingPage "714" @default.
• W2056556864 startingPage "703" @default.
• W2056556864 abstract "Recent reports suggest that the nuclear envelope possesses specific ion transport mechanisms that regulate the electrolyte concentrations within the nucleoplasm and perinuclear space. In this work, intact nuclei were isolated from sheep cardiac cells. After chromatin digestion, the nuclear envelopes were sonicated and four nuclear vesicle populations were separated by sucrose step gradients (SF1-SF4). These fractions were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their protein content was analyzed by Western blot, using lamin and SEC 61 antibodies. The lamins, which are associated with the inner nuclear membrane, were present in three fractions, SF2, SF3, and SF4, with a lower amount in SF2. The SEC 61 protein, a marker of the rough endoplasmic reticulum, was detected in small amounts in SF1 and SF2. Upon fusion of vesicles into bilayers, the activities of nuclear ionic channels were recorded in 50 mM trans/250 mM cis KCl or CsCl, pH 7.2. Two types of Cl- selective channels were recorded: a large conducting 150-180-pS channel displaying substates, and a low conducting channel of 30 pS. They were both spontaneously active into bilayers, and their open probability was poorly voltage dependent at negative voltages. Retinoic acid (10(-8) M) increases the po of the large Cl- conducting channel, whereas ATP modifies the kinetics of the low conductance anion selective channel. Our data also suggest that this anionic channel is mainly present in the SF3 and SF4 population. The presence of a 181 +/- 10 pS cation-selective channel was consistently observed in the SF2 population. The behavior of this channel was voltage dependent in the voltage range -80 to +60 mV. Furthermore, we report for the first time the activity of a channel exclusively present in the SF3 and SF4 fractions, shown to contain mainly inner membrane vesicles. This cation selective channel displays a 75-pS conductance in 50 mM trans/250 mM cis K-gluconate. It is concluded that the bilayer reconstitution technique is an attractive approach to studying the electrophysiological properties of the inner and outer membranes of the nuclear envelope." @default.
• W2056556864 created "2016-06-24" @default.
• W2056556864 creator A5023386783 @default.
• W2056556864 creator A5036775454 @default.
• W2056556864 creator A5053943131 @default.
• W2056556864 creator A5074145533 @default.
• W2056556864 creator A5088723836 @default.
• W2056556864 date "1996-02-01" @default.
• W2056556864 modified "2023-09-27" @default.
• W2056556864 title "Reconstitution of ionic channels from inner and outer membranes of mammalian cardiac nuclei" @default.
• W2056556864 cites W113046133 @default.
• W2056556864 cites W1439548706 @default.
• W2056556864 cites W1484015205 @default.
• W2056556864 cites W1505536548 @default.
• W2056556864 cites W1508288896 @default.
• W2056556864 cites W1584619663 @default.
• W2056556864 cites W1602198467 @default.
• W2056556864 cites W1775749144 @default.
• W2056556864 cites W1784106556 @default.
• W2056556864 cites W1788868849 @default.
• W2056556864 cites W1845085723 @default.
• W2056556864 cites W1968492034 @default.
• W2056556864 cites W1972045947 @default.
• W2056556864 cites W1980243029 @default.
• W2056556864 cites W1986311952 @default.
• W2056556864 cites W1988187116 @default.
• W2056556864 cites W1988818674 @default.
• W2056556864 cites W1989502344 @default.
• W2056556864 cites W1993222258 @default.
• W2056556864 cites W1994507975 @default.
• W2056556864 cites W1998806475 @default.
• W2056556864 cites W2004624771 @default.
• W2056556864 cites W2006899992 @default.
• W2056556864 cites W2020784937 @default.
• W2056556864 cites W2035091883 @default.
• W2056556864 cites W2041389127 @default.
• W2056556864 cites W2045186361 @default.
• W2056556864 cites W2059845021 @default.
• W2056556864 cites W2066433778 @default.
• W2056556864 cites W2073387875 @default.
• W2056556864 cites W2073391946 @default.
• W2056556864 cites W2075791686 @default.
• W2056556864 cites W2076339054 @default.
• W2056556864 cites W2077111582 @default.
• W2056556864 cites W2078566041 @default.
• W2056556864 cites W2082833702 @default.
• W2056556864 cites W2084069576 @default.
• W2056556864 cites W2100971576 @default.
• W2056556864 cites W2110499044 @default.
• W2056556864 cites W2115395655 @default.
• W2056556864 cites W2122080661 @default.
• W2056556864 cites W2122341536 @default.
• W2056556864 cites W2122947137 @default.
• W2056556864 cites W2136710801 @default.
• W2056556864 cites W2139907477 @default.
• W2056556864 cites W2141300290 @default.
• W2056556864 cites W2147182875 @default.
• W2056556864 cites W4249808084 @default.
• W2056556864 doi "https://doi.org/10.1016/s0006-3495(96)79610-8" @default.
• W2056556864 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1224970" @default.
• W2056556864 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8789087" @default.
• W2056556864 hasPublicationYear "1996" @default.
• W2056556864 type Work @default.
• W2056556864 sameAs 2056556864 @default.
• W2056556864 citedByCount "38" @default.
• W2056556864 countsByYear W20565568642012 @default.
• W2056556864 countsByYear W20565568642013 @default.
• W2056556864 countsByYear W20565568642014 @default.
• W2056556864 countsByYear W20565568642017 @default.
• W2056556864 countsByYear W20565568642022 @default.
• W2056556864 crossrefType "journal-article" @default.
• W2056556864 hasAuthorship W2056556864A5023386783 @default.
• W2056556864 hasAuthorship W2056556864A5036775454 @default.
• W2056556864 hasAuthorship W2056556864A5053943131 @default.
• W2056556864 hasAuthorship W2056556864A5074145533 @default.
• W2056556864 hasAuthorship W2056556864A5088723836 @default.
• W2056556864 hasBestOaLocation W20565568641 @default.
• W2056556864 hasConcept C104317684 @default.
• W2056556864 hasConcept C112952253 @default.
• W2056556864 hasConcept C12554922 @default.
• W2056556864 hasConcept C130316041 @default.
• W2056556864 hasConcept C144024400 @default.
• W2056556864 hasConcept C149923435 @default.
• W2056556864 hasConcept C158617107 @default.
• W2056556864 hasConcept C179093185 @default.
• W2056556864 hasConcept C183873130 @default.
• W2056556864 hasConcept C185592680 @default.
• W2056556864 hasConcept C190062978 @default.
• W2056556864 hasConcept C2908647359 @default.
• W2056556864 hasConcept C41625074 @default.
• W2056556864 hasConcept C55493867 @default.
• W2056556864 hasConcept C65871279 @default.
• W2056556864 hasConcept C86803240 @default.
• W2056556864 hasConceptScore W2056556864C104317684 @default.
• W2056556864 hasConceptScore W2056556864C112952253 @default.
• W2056556864 hasConceptScore W2056556864C12554922 @default.
• W2056556864 hasConceptScore W2056556864C130316041 @default.
• W2056556864 hasConceptScore W2056556864C144024400 @default.

• next