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• W2056565088 abstract "A Zn- and pH-buffered medium was used to study the accumulation of Zn in batch-cultured algae (Selenastrum capricornutum Printz.). All experiments were carried out using 65Zn radiotracers, which were measured by γ-ray spectrometry. Zinc was applied in the presence of nitrilotriacetic acid (NTA), leading to free Zn2+ concentrations ranging from 4·2 × 10−11 to 1·6 × 10−7 mol litre−1. During the 75-h experiments, the rates of Zn influx and efflux were determined, the latter with full consideration for algal growth rates. Algal growth stopped completely at a free Zn2+ concentration of 1·6 × 10−8 mol litre−1. Initial Zn binding was determined and, based on free Zn2+ concentrations, presented by the apparent dissociation constant Kdiss and capacity C as 4·6 × 10−9 mol litre−1 and 19·8 × 106 mol gDW−1, respectively. The rate constant of Zn efflux could be calculated as a constant 0·021 ± 0·003 h−1, irrespective of the Zn concentrations applied. The rates of Zn influx were expressed by the Vmax and KM values as 3·3 × 10−10 mol gDW−1 s−1 and 6·3 × 10−9 mol litre−1, respectively. These values, which are based on applied free Zn2+ concentrations, are compatible with the operation of a high-affinity low-concentration carrier mechanism. The results suggest that, under the conditions applied, free Zn2+ may be the most relevant Zn species for Zn uptake; furthermore, relative growth rate may be regarded as a relatively sensitive signal for Zn stress circumstances." @default.
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• W2056565088 date "1995-01-01" @default.
• W2056565088 modified "2023-10-16" @default.
• W2056565088 title "On the uptake and release of zinc (65Zn) in the growing alga Selenastrum capricornutum Printz" @default.
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• W2056565088 doi "https://doi.org/10.1016/0269-7491(95)91051-l" @default.
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• W2056565088 hasPublicationYear "1995" @default.
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