FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056565816> ?p ?o ?g. }

• W2056565816 endingPage "128" @default.
• W2056565816 startingPage "121" @default.
• W2056565816 abstract "The 70-kDa heat-shock cognate protein (HSC70), a constitutively expressed protein in mammalian cells, plays a major role in several cellular processes such as protein folding and assembly, uncoating of clathrin-coated vesicles and transport of protein through membranes. HSC70 has been overexpressed in Escherichia coli in a soluble form using a designed two-cistron expression vector, and purified to homogeneity in a two-step procedure involving ion-exchange and affinity chromatography. Up to 20 mg of pure protein could be obtained from 11 of cell culture. Amino-terminal sequencing of the recombinant protein gives the expected sequence, and non-denaturing gel electrophoresis as well as gel filtration analysis reveal the presence of self-associating species that could be dissociated by ATP. Crosslinking studies confirm the presence of multiple species and the dissociating effect of ATP. Temperatures above 42 degrees C induce the aggregation of HSC70; ATP shifts this effect to higher temperatures. The recombinant protein displays a low intrinsic ATPase activity that can be stimulated about threefold by binding to apocytochrome c, a permanently unfolded protein, while native cytochrome c has no effect on the ATPase activity indicating that recombinant HSC70 binds specifically unfolded protein but not their native counterpart. Thus, efficient production of recombinant HSC70 having structural and functional properties comparable to those of the natural protein could be achieved, thereby allowing the molecular basis of the chaperone function and its regulation through ATP hydrolysis to be probed." @default.
• W2056565816 created "2016-06-24" @default.
• W2056565816 creator A5001338747 @default.
• W2056565816 creator A5039327076 @default.
• W2056565816 creator A5042131361 @default.
• W2056565816 creator A5043412497 @default.
• W2056565816 creator A5077593598 @default.
• W2056565816 date "1994-04-01" @default.
• W2056565816 modified "2023-09-25" @default.
• W2056565816 title "Overexpression in Escherichia coli, purification and characterization of the molecular chaperone HSC70" @default.
• W2056565816 cites W1490139598 @default.
• W2056565816 cites W1518828679 @default.
• W2056565816 cites W1529321087 @default.
• W2056565816 cites W1539678880 @default.
• W2056565816 cites W1545540883 @default.
• W2056565816 cites W1555021163 @default.
• W2056565816 cites W1558079148 @default.
• W2056565816 cites W1574522151 @default.
• W2056565816 cites W1586954919 @default.
• W2056565816 cites W1593791512 @default.
• W2056565816 cites W1685892021 @default.
• W2056565816 cites W1769448430 @default.
• W2056565816 cites W1857497956 @default.
• W2056565816 cites W1873199501 @default.
• W2056565816 cites W1958996074 @default.
• W2056565816 cites W1964652518 @default.
• W2056565816 cites W1968155837 @default.
• W2056565816 cites W1971800526 @default.
• W2056565816 cites W1977357027 @default.
• W2056565816 cites W1977388184 @default.
• W2056565816 cites W1989054182 @default.
• W2056565816 cites W1989350796 @default.
• W2056565816 cites W1990650142 @default.
• W2056565816 cites W1994022052 @default.
• W2056565816 cites W2000472969 @default.
• W2056565816 cites W2005383788 @default.
• W2056565816 cites W2013944211 @default.
• W2056565816 cites W2023759182 @default.
• W2056565816 cites W2023966699 @default.
• W2056565816 cites W2026998495 @default.
• W2056565816 cites W2029306713 @default.
• W2056565816 cites W2034943952 @default.
• W2056565816 cites W2045496227 @default.
• W2056565816 cites W2051643639 @default.
• W2056565816 cites W2051793369 @default.
• W2056565816 cites W2057337811 @default.
• W2056565816 cites W2061888900 @default.
• W2056565816 cites W2062686601 @default.
• W2056565816 cites W2064977032 @default.
• W2056565816 cites W2067708969 @default.
• W2056565816 cites W2070692986 @default.
• W2056565816 cites W2072744250 @default.
• W2056565816 cites W2085021338 @default.
• W2056565816 cites W2087434802 @default.
• W2056565816 cites W2090317414 @default.
• W2056565816 cites W2091288867 @default.
• W2056565816 cites W2092198909 @default.
• W2056565816 cites W2092713786 @default.
• W2056565816 cites W2098912888 @default.
• W2056565816 cites W2100837269 @default.
• W2056565816 cites W2125253481 @default.
• W2056565816 cites W2138270253 @default.
• W2056565816 cites W2164179462 @default.
• W2056565816 cites W2178910979 @default.
• W2056565816 cites W2498072673 @default.
• W2056565816 cites W4232707708 @default.
• W2056565816 cites W4237068377 @default.
• W2056565816 cites W4239254452 @default.
• W2056565816 cites W4240454981 @default.
• W2056565816 cites W4247255621 @default.
• W2056565816 cites W4249988281 @default.
• W2056565816 cites W628204631 @default.
• W2056565816 doi "https://doi.org/10.1111/j.1432-1033.1994.tb18720.x" @default.
• W2056565816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8168501" @default.
• W2056565816 hasPublicationYear "1994" @default.
• W2056565816 type Work @default.
• W2056565816 sameAs 2056565816 @default.
• W2056565816 citedByCount "21" @default.
• W2056565816 countsByYear W20565658162015 @default.
• W2056565816 countsByYear W20565658162018 @default.
• W2056565816 crossrefType "journal-article" @default.
• W2056565816 hasAuthorship W2056565816A5001338747 @default.
• W2056565816 hasAuthorship W2056565816A5039327076 @default.
• W2056565816 hasAuthorship W2056565816A5042131361 @default.
• W2056565816 hasAuthorship W2056565816A5043412497 @default.
• W2056565816 hasAuthorship W2056565816A5077593598 @default.
• W2056565816 hasBestOaLocation W20565658161 @default.
• W2056565816 hasConcept C104317684 @default.
• W2056565816 hasConcept C141315368 @default.
• W2056565816 hasConcept C142724271 @default.
• W2056565816 hasConcept C153911025 @default.
• W2056565816 hasConcept C179247698 @default.
• W2056565816 hasConcept C181199279 @default.
• W2056565816 hasConcept C205260736 @default.
• W2056565816 hasConcept C23265538 @default.
• W2056565816 hasConcept C2775962898 @default.
• W2056565816 hasConcept C40767141 @default.
• W2056565816 hasConcept C547475151 @default.

• next