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- W2056580780 abstract "Abstract Partial purification of cytoplasmic-polyhedrosis virus (CPV) of the silkworm was previously undertaken by Hukuhara and Hashimoto. Further purification of virus-containing suspensions was tried by centrifugation in a sucrose density gradient after fluorocarbon treatments, and two zones or fractions designated “top” and “bottom”, having sedimentation coefficient of approximately 260S and 415S, were obtained. The ultraviolet absorption spectra of the bottom zone showed typical nucleoprotein with a maximum at 260 mμ and a minimum at 240 mμ, but in the top zone some contamination of the protein component was observed. Infectivity was confined to the bottom zone, and fluorocarbon did not affect the infectivity by the two treatments. Particles from the bottom zone were typical icosahedral particles but in the top the shape was irregular and may represent empty “shells.” The evidence indicates that the particles in the bottom zone obtained by this method are pure cytoplasmic-polyhedrosis virus particles. The content of CPV particles was related to absorbance at 260 mμ, and it was clear that the 3.7 absorbance unit represented 1 mg CPV/ml." @default.
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- W2056580780 date "1969-03-01" @default.
- W2056580780 modified "2023-09-27" @default.
- W2056580780 title "Purification of a cytoplasmic-polyhedrosis virus of the silkworm, Bombyx mori" @default.
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- W2056580780 doi "https://doi.org/10.1016/0022-2011(69)90222-5" @default.
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