Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056584507> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2056584507 endingPage "222" @default.
- W2056584507 startingPage "213" @default.
- W2056584507 abstract "A rapid, sensitive and specific analytical method has been developed and validated to quantify the collagenase inhibitor N2-(2(5)-[(hydroxycarbamoyl)methyl)-4-methylvaleryl]-N1,3-dimethyl-l-valin-amide (I) and its major metabolite (II) from plasma and urine. This method employs an automated solid-phase extraction procedure to isolate the analytes and the internal standard from the biological matrix. Reconstituted extracts were analyzed by HPLC-ionspray MS-MS. Chromatography was performed on a 4.6 mm I.D. reversed-phase guard column. The retention times of the analytes and the internal standard were approximately 1.3 min. The assay has a limit of quantification of 5 ng/ml plasma and a limit of detection of 1 ng/ml, based on 100-μl plasma aliquots. No sample-drying step was required. The standard curves were linear from 5 to 5000 ng/ml using weighted linear regression analysis (1y2). The intra- and inter-assay precision were better than ±10% with intra- and inter-assay accuracies between i5 and 105%. This new HPLC-MS-MS assay procedure for I and II in plasma and urine has proven to be specific, sensitive, accurate and robust, and is being used routinely for the analysis of I in pre-clinical and clinical trial samples. Up to 200 unknowns may be analyzed each 24 h per analyst." @default.
- W2056584507 created "2016-06-24" @default.
- W2056584507 creator A5009582654 @default.
- W2056584507 creator A5048116821 @default.
- W2056584507 creator A5078992572 @default.
- W2056584507 date "1995-11-01" @default.
- W2056584507 modified "2023-10-14" @default.
- W2056584507 title "Rapid quantitative determination of a collagenase inhibitor and its major metabolite by on-line liquid chromatography with ionspray tandem mass spectrometric detection" @default.
- W2056584507 cites W1990256494 @default.
- W2056584507 cites W2003570147 @default.
- W2056584507 cites W2054101123 @default.
- W2056584507 cites W2063914793 @default.
- W2056584507 cites W2068436137 @default.
- W2056584507 cites W2132327529 @default.
- W2056584507 cites W2133692536 @default.
- W2056584507 doi "https://doi.org/10.1016/0378-4347(95)00270-2" @default.
- W2056584507 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8611955" @default.
- W2056584507 hasPublicationYear "1995" @default.
- W2056584507 type Work @default.
- W2056584507 sameAs 2056584507 @default.
- W2056584507 citedByCount "11" @default.
- W2056584507 countsByYear W20565845072019 @default.
- W2056584507 crossrefType "journal-article" @default.
- W2056584507 hasAuthorship W2056584507A5009582654 @default.
- W2056584507 hasAuthorship W2056584507A5048116821 @default.
- W2056584507 hasAuthorship W2056584507A5078992572 @default.
- W2056584507 hasConcept C10390740 @default.
- W2056584507 hasConcept C119128265 @default.
- W2056584507 hasConcept C162356407 @default.
- W2056584507 hasConcept C179998833 @default.
- W2056584507 hasConcept C185592680 @default.
- W2056584507 hasConcept C2777477808 @default.
- W2056584507 hasConcept C2778576202 @default.
- W2056584507 hasConcept C31827203 @default.
- W2056584507 hasConcept C43617362 @default.
- W2056584507 hasConcept C55493867 @default.
- W2056584507 hasConcept C70784841 @default.
- W2056584507 hasConcept C81426234 @default.
- W2056584507 hasConcept C95986675 @default.
- W2056584507 hasConceptScore W2056584507C10390740 @default.
- W2056584507 hasConceptScore W2056584507C119128265 @default.
- W2056584507 hasConceptScore W2056584507C162356407 @default.
- W2056584507 hasConceptScore W2056584507C179998833 @default.
- W2056584507 hasConceptScore W2056584507C185592680 @default.
- W2056584507 hasConceptScore W2056584507C2777477808 @default.
- W2056584507 hasConceptScore W2056584507C2778576202 @default.
- W2056584507 hasConceptScore W2056584507C31827203 @default.
- W2056584507 hasConceptScore W2056584507C43617362 @default.
- W2056584507 hasConceptScore W2056584507C55493867 @default.
- W2056584507 hasConceptScore W2056584507C70784841 @default.
- W2056584507 hasConceptScore W2056584507C81426234 @default.
- W2056584507 hasConceptScore W2056584507C95986675 @default.
- W2056584507 hasIssue "2" @default.
- W2056584507 hasLocation W20565845071 @default.
- W2056584507 hasLocation W20565845072 @default.
- W2056584507 hasOpenAccess W2056584507 @default.
- W2056584507 hasPrimaryLocation W20565845071 @default.
- W2056584507 hasRelatedWork W1503327256 @default.
- W2056584507 hasRelatedWork W1981462688 @default.
- W2056584507 hasRelatedWork W2041637299 @default.
- W2056584507 hasRelatedWork W2089646276 @default.
- W2056584507 hasRelatedWork W2750173713 @default.
- W2056584507 hasRelatedWork W2971174350 @default.
- W2056584507 hasRelatedWork W3025930938 @default.
- W2056584507 hasRelatedWork W4213451887 @default.
- W2056584507 hasRelatedWork W4225004660 @default.
- W2056584507 hasRelatedWork W4302013369 @default.
- W2056584507 hasVolume "673" @default.
- W2056584507 isParatext "false" @default.
- W2056584507 isRetracted "false" @default.
- W2056584507 magId "2056584507" @default.
- W2056584507 workType "article" @default.