Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056673488> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2056673488 endingPage "752" @default.
- W2056673488 startingPage "745" @default.
- W2056673488 abstract "The effect of DnaB helicase on the initiation specificity of primase was studied biochemically using a series of single-stranded DNA templates in which each nucleotide of the trinucleotide d(CTG) initiation sequence was systematically varied. DnaB helicase accelerated the rate of primer syntheisis, prevented overlong primers from forming and decreased the initiation specificity of primase. In the presence of DnaB helicase, all trinucleotides could serve as the primer initiation site although there was a distinct preference for d(CAG). These data may explain the high chromosomal prevalence of octanucleotides containing CTG on the leading strand and its complement CAG on the lagging strand. The specificity of DnaB helicase places it on the lagging strand template where it stimulates the initiation of Okazaki fragment synthesis. In the absence of DnaB helicase, primase preferentially primed the d(CTG) template. In the presence of DnaB helicase, the initiation preference was not only altered but also the preferred initiating nucleotide was found to be GTP rather than ATP, for both the d(CTG) and the d(CAG) templates. This suggested that the specificity of primase for the d(CTG) initiation trinucleotide was predominantly unaffected in the absence of DnaB helicase on short ssDNA templates, whereas in conjunction with DnaB helicase, the specificity was altered and this alteration has significant implications in the replication of Escherichia coli chromosome in vivo." @default.
- W2056673488 created "2016-06-24" @default.
- W2056673488 creator A5017066622 @default.
- W2056673488 creator A5049940870 @default.
- W2056673488 date "2000-01-04" @default.
- W2056673488 modified "2023-09-29" @default.
- W2056673488 title "DnaB Helicase Affects the Initiation Specifity of <i>Escherichia coli</i> Primase on Single-Stranded DNA Templates" @default.
- W2056673488 cites W1512516275 @default.
- W2056673488 cites W1532280716 @default.
- W2056673488 cites W1561140582 @default.
- W2056673488 cites W2006667780 @default.
- W2056673488 cites W2017398658 @default.
- W2056673488 cites W2109024607 @default.
- W2056673488 cites W2140220147 @default.
- W2056673488 cites W22874528 @default.
- W2056673488 doi "https://doi.org/10.1021/bi991555d" @default.
- W2056673488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10651640" @default.
- W2056673488 hasPublicationYear "2000" @default.
- W2056673488 type Work @default.
- W2056673488 sameAs 2056673488 @default.
- W2056673488 citedByCount "35" @default.
- W2056673488 countsByYear W20566734882012 @default.
- W2056673488 countsByYear W20566734882013 @default.
- W2056673488 countsByYear W20566734882014 @default.
- W2056673488 countsByYear W20566734882015 @default.
- W2056673488 countsByYear W20566734882016 @default.
- W2056673488 countsByYear W20566734882017 @default.
- W2056673488 crossrefType "journal-article" @default.
- W2056673488 hasAuthorship W2056673488A5017066622 @default.
- W2056673488 hasAuthorship W2056673488A5049940870 @default.
- W2056673488 hasConcept C104317684 @default.
- W2056673488 hasConcept C116107829 @default.
- W2056673488 hasConcept C153911025 @default.
- W2056673488 hasConcept C156719811 @default.
- W2056673488 hasConcept C161223559 @default.
- W2056673488 hasConcept C188192080 @default.
- W2056673488 hasConcept C194830204 @default.
- W2056673488 hasConcept C54355233 @default.
- W2056673488 hasConcept C552990157 @default.
- W2056673488 hasConcept C58167604 @default.
- W2056673488 hasConcept C67705224 @default.
- W2056673488 hasConcept C73573662 @default.
- W2056673488 hasConcept C86803240 @default.
- W2056673488 hasConceptScore W2056673488C104317684 @default.
- W2056673488 hasConceptScore W2056673488C116107829 @default.
- W2056673488 hasConceptScore W2056673488C153911025 @default.
- W2056673488 hasConceptScore W2056673488C156719811 @default.
- W2056673488 hasConceptScore W2056673488C161223559 @default.
- W2056673488 hasConceptScore W2056673488C188192080 @default.
- W2056673488 hasConceptScore W2056673488C194830204 @default.
- W2056673488 hasConceptScore W2056673488C54355233 @default.
- W2056673488 hasConceptScore W2056673488C552990157 @default.
- W2056673488 hasConceptScore W2056673488C58167604 @default.
- W2056673488 hasConceptScore W2056673488C67705224 @default.
- W2056673488 hasConceptScore W2056673488C73573662 @default.
- W2056673488 hasConceptScore W2056673488C86803240 @default.
- W2056673488 hasIssue "4" @default.
- W2056673488 hasLocation W20566734881 @default.
- W2056673488 hasLocation W20566734882 @default.
- W2056673488 hasOpenAccess W2056673488 @default.
- W2056673488 hasPrimaryLocation W20566734881 @default.
- W2056673488 hasRelatedWork W107673453 @default.
- W2056673488 hasRelatedWork W2001823184 @default.
- W2056673488 hasRelatedWork W2005699935 @default.
- W2056673488 hasRelatedWork W2050030747 @default.
- W2056673488 hasRelatedWork W2052508338 @default.
- W2056673488 hasRelatedWork W2056058186 @default.
- W2056673488 hasRelatedWork W2110576616 @default.
- W2056673488 hasRelatedWork W2417446516 @default.
- W2056673488 hasRelatedWork W2926621447 @default.
- W2056673488 hasRelatedWork W332628024 @default.
- W2056673488 hasVolume "39" @default.
- W2056673488 isParatext "false" @default.
- W2056673488 isRetracted "false" @default.
- W2056673488 magId "2056673488" @default.
- W2056673488 workType "article" @default.