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- W2056698634 abstract "A method is described for the quantitative analysis and preparative isolation of N-[N-methyl-N-(9-β-ribofuranosylpurin-6-yl)carbamoyl]threonine (mt6A), a rare modified nucleoside constituent of transfer RNA. This method is based on the selective retention of mt6A and its parent compound, t6A, on Dowex-1 at pH 7.8, allowing these two nucleosides to be readily concentrated from the mixture of nucleosides resulting when tRNA is hydrolyzed by a combination of snake venom enzymes and E. coli alkaline phosphatase. The content of mt6A in wheat embryo and E. coli tRNA was found to be about 0.025 mole %, which is roughly one-tenth the t6A content of the tRNA of these two organisms. Since this indicates that only about 1 in 50 chains can contain a residue of mt6A, this nucleoside may be confined to a single isoaccepting species of transfer RNA in both E. coli and wheat embryo. No mt6A could be detected in either baker's or brewer's yeast tRNA by the method described. Either mt6A is entirely absent from yeast tRNA or it occurs in a form which does not adsorb to Dowex-1 during fractionation of hydrolysates of yeast tRNA. No t6A or mt6A could be detected in the 18 S + 26 S ribosomal ribonucleates of wheat embryo." @default.
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- W2056698634 date "1974-11-01" @default.
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- W2056698634 title "A method for the quantitative analysis and preparative isolation of N-[N-methyl-N-(9-β-d-ribofuranosylpurin-6-yl)carbamoyl]threonine—A modified nucleoside present in transfer RNA" @default.
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- W2056698634 doi "https://doi.org/10.1016/0003-2697(74)90370-4" @default.
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