FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056702922> ?p ?o ?g. }

• W2056702922 abstract "Guanine nucleotide binding protein (G-protein) coupled receptors are involved in smooth muscle cell proliferation, but the role of G-proteins in arterial intimal hyperplasia has not been defined. This study examines the expression of G-proteins in the developing intimal hyperplasia after balloon injury of the rat carotid artery and specifically tests the hypothesis that the pertussis toxin sensitive G(i) G-protein subunit plays a role in the initiation of intimal hyperplasia.In vitro responses to serum stimulation (10% fetal bovine serum) were examined in the presence and absence of pertussis toxin (PTx). After a standard balloon injury in male Sprague-Dawley rats, the expression of G-protein subunits (alpha(o), alpha(i), alpha(q), alpha(s), and betagamma) was determined by means of Western blotting in the first 28 days. Thereafter, a second set of animals was allocated to control and PTx-treated (a Galpha(i) inhibitor; 500 ng/mL in an externally applied 30% pluronic gel) groups. Smooth muscle cell proliferation was estimated by means of thymidine analogue 5-bromo-2' deoxyuridine incorporation 2 days after injury, and vessel dimensions were determined by means of videomorphometry 14 days after injury.There was inhibition of DNA synthesis and smooth muscle cell proliferation in response to serum with an IC(50) of 100 ng/mL. Three days after balloon injury, there was an increase in Galpha(i3) expression, which decreased at days 7, 14, and 28, compared with the uninjured carotid. Galpha(q) expression increased in a time-dependent manner. There was a marked time-dependent increase in Gbetagamma in the 28 days. Galpha(i2) and Galpha(s) isoforms (45 and 52 kDa) did not change significantly with time. There was no major change in Galpha(i1) and Galpha(o) in the study period. At 14 days, PTx treatment reduced intimal hyperplasia by 52% (63 +/- 4 microm vs. 30 +/- 5 microm, control vs. PTx; P <.001). Medial smooth muscle cell proliferation at day 2 was decreased in the PTx group, compared with that in the gel-coated group (15% +/- 2% and 26% +/- 3%; P = .02).After balloon injury, there is a time-dependent increase in G-protein expression, which is subunit specific. Activation of PTx sensitive G-proteins (Galpha(i)) is involved during the initiation of intimal hyperplasia after arterial injury, and their inhibition results in a decrease in early medial cell proliferation. This acute interruption of G(i) signaling produces a long-term decrease in intimal hyperplasia." @default.
• W2056702922 created "2016-06-24" @default.
• W2056702922 creator A5006131577 @default.
• W2056702922 creator A5006673395 @default.
• W2056702922 creator A5027120100 @default.
• W2056702922 creator A5033247608 @default.
• W2056702922 creator A5064950593 @default.
• W2056702922 creator A5066362644 @default.
• W2056702922 date "2001-02-01" @default.
• W2056702922 modified "2023-09-27" @default.
• W2056702922 title "G-protein expression and intimal hyperplasia after arterial injury: A role for Gαi proteins" @default.
• W2056702922 cites W1526609249 @default.
• W2056702922 cites W1968692561 @default.
• W2056702922 cites W1970517621 @default.
• W2056702922 cites W1971260370 @default.
• W2056702922 cites W1972168628 @default.
• W2056702922 cites W1975915718 @default.
• W2056702922 cites W1978274173 @default.
• W2056702922 cites W1980480613 @default.
• W2056702922 cites W1987108104 @default.
• W2056702922 cites W1990581298 @default.
• W2056702922 cites W1992307459 @default.
• W2056702922 cites W2000352826 @default.
• W2056702922 cites W2000862518 @default.
• W2056702922 cites W2015219342 @default.
• W2056702922 cites W2022142677 @default.
• W2056702922 cites W2023143614 @default.
• W2056702922 cites W2034070845 @default.
• W2056702922 cites W2034487226 @default.
• W2056702922 cites W2040216156 @default.
• W2056702922 cites W2041104433 @default.
• W2056702922 cites W2048527391 @default.
• W2056702922 cites W2049779218 @default.
• W2056702922 cites W2070710936 @default.
• W2056702922 cites W2080875376 @default.
• W2056702922 cites W2085415643 @default.
• W2056702922 cites W2088823687 @default.
• W2056702922 cites W2090231105 @default.
• W2056702922 cites W2099736471 @default.
• W2056702922 cites W2110983953 @default.
• W2056702922 cites W2112225171 @default.
• W2056702922 cites W2135918821 @default.
• W2056702922 cites W2160146943 @default.
• W2056702922 cites W2076121635 @default.
• W2056702922 doi "https://doi.org/10.1067/mva.2001.111748" @default.
• W2056702922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11174797" @default.
• W2056702922 hasPublicationYear "2001" @default.
• W2056702922 type Work @default.
• W2056702922 sameAs 2056702922 @default.
• W2056702922 citedByCount "7" @default.
• W2056702922 countsByYear W20567029222012 @default.
• W2056702922 countsByYear W20567029222013 @default.
• W2056702922 countsByYear W20567029222015 @default.
• W2056702922 crossrefType "journal-article" @default.
• W2056702922 hasAuthorship W2056702922A5006131577 @default.
• W2056702922 hasAuthorship W2056702922A5006673395 @default.
• W2056702922 hasAuthorship W2056702922A5027120100 @default.
• W2056702922 hasAuthorship W2056702922A5033247608 @default.
• W2056702922 hasAuthorship W2056702922A5064950593 @default.
• W2056702922 hasAuthorship W2056702922A5066362644 @default.
• W2056702922 hasBestOaLocation W20567029221 @default.
• W2056702922 hasConcept C104317684 @default.
• W2056702922 hasConcept C126322002 @default.
• W2056702922 hasConcept C134018914 @default.
• W2056702922 hasConcept C1491633281 @default.
• W2056702922 hasConcept C16685009 @default.
• W2056702922 hasConcept C170493617 @default.
• W2056702922 hasConcept C2776415932 @default.
• W2056702922 hasConcept C2777339539 @default.
• W2056702922 hasConcept C2777562237 @default.
• W2056702922 hasConcept C2778283817 @default.
• W2056702922 hasConcept C2778583881 @default.
• W2056702922 hasConcept C2779395532 @default.
• W2056702922 hasConcept C2779404806 @default.
• W2056702922 hasConcept C2779751240 @default.
• W2056702922 hasConcept C2992686903 @default.
• W2056702922 hasConcept C49773422 @default.
• W2056702922 hasConcept C55493867 @default.
• W2056702922 hasConcept C62112901 @default.
• W2056702922 hasConcept C71924100 @default.
• W2056702922 hasConcept C80631254 @default.
• W2056702922 hasConcept C85265743 @default.
• W2056702922 hasConcept C86803240 @default.
• W2056702922 hasConceptScore W2056702922C104317684 @default.
• W2056702922 hasConceptScore W2056702922C126322002 @default.
• W2056702922 hasConceptScore W2056702922C134018914 @default.
• W2056702922 hasConceptScore W2056702922C1491633281 @default.
• W2056702922 hasConceptScore W2056702922C16685009 @default.
• W2056702922 hasConceptScore W2056702922C170493617 @default.
• W2056702922 hasConceptScore W2056702922C2776415932 @default.
• W2056702922 hasConceptScore W2056702922C2777339539 @default.
• W2056702922 hasConceptScore W2056702922C2777562237 @default.
• W2056702922 hasConceptScore W2056702922C2778283817 @default.
• W2056702922 hasConceptScore W2056702922C2778583881 @default.
• W2056702922 hasConceptScore W2056702922C2779395532 @default.
• W2056702922 hasConceptScore W2056702922C2779404806 @default.
• W2056702922 hasConceptScore W2056702922C2779751240 @default.
• W2056702922 hasConceptScore W2056702922C2992686903 @default.
• W2056702922 hasConceptScore W2056702922C49773422 @default.
• W2056702922 hasConceptScore W2056702922C55493867 @default.

• next