Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056790168> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2056790168 endingPage "157" @default.
- W2056790168 startingPage "149" @default.
- W2056790168 abstract "The measurement of cytokines in plasma and other fluids often requires the use of an enzyme-linked immunosorbant assay (ELISA). In the research environment, a valuable assay is one that yields reliable results in the shortest amount of time for the least cost. To achieve this goal, a protocol has been outlined to develop sandwich ELISAs for cytokines using commercial antibodies. These guidelines for ELISA development include selecting antibody concentrations, choosing an appropriate buffer, reducing plasma interference and evaluating the optimal length for incubation periods. In addition, the protocol for a rapid IL-6 ELISA is presented. This ELISA allows measurement of IL-6 in a reduced amount of time by raising the concentration of antibodies used and increasing the temperature for incubation. By following the guidelines presented, cost-effective, cytokine ELISAs can be developed that yield low background, detect a wide range of concentrations, and are suitable for use in the research setting." @default.
- W2056790168 created "2016-06-24" @default.
- W2056790168 creator A5008863485 @default.
- W2056790168 creator A5039409631 @default.
- W2056790168 creator A5083968353 @default.
- W2056790168 date "2001-09-01" @default.
- W2056790168 modified "2023-10-07" @default.
- W2056790168 title "Development and optimization of cytokine ELISAs using commercial antibody pairs" @default.
- W2056790168 cites W1496300019 @default.
- W2056790168 cites W1993313447 @default.
- W2056790168 cites W2004697169 @default.
- W2056790168 cites W2028746410 @default.
- W2056790168 cites W2042644034 @default.
- W2056790168 cites W2097122290 @default.
- W2056790168 doi "https://doi.org/10.1016/s0022-1759(01)00419-7" @default.
- W2056790168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11470295" @default.
- W2056790168 hasPublicationYear "2001" @default.
- W2056790168 type Work @default.
- W2056790168 sameAs 2056790168 @default.
- W2056790168 citedByCount "108" @default.
- W2056790168 countsByYear W20567901682012 @default.
- W2056790168 countsByYear W20567901682013 @default.
- W2056790168 countsByYear W20567901682014 @default.
- W2056790168 countsByYear W20567901682015 @default.
- W2056790168 countsByYear W20567901682016 @default.
- W2056790168 countsByYear W20567901682017 @default.
- W2056790168 countsByYear W20567901682018 @default.
- W2056790168 countsByYear W20567901682019 @default.
- W2056790168 countsByYear W20567901682021 @default.
- W2056790168 countsByYear W20567901682022 @default.
- W2056790168 countsByYear W20567901682023 @default.
- W2056790168 crossrefType "journal-article" @default.
- W2056790168 hasAuthorship W2056790168A5008863485 @default.
- W2056790168 hasAuthorship W2056790168A5039409631 @default.
- W2056790168 hasAuthorship W2056790168A5083968353 @default.
- W2056790168 hasConcept C142724271 @default.
- W2056790168 hasConcept C159654299 @default.
- W2056790168 hasConcept C185592680 @default.
- W2056790168 hasConcept C203014093 @default.
- W2056790168 hasConcept C204787440 @default.
- W2056790168 hasConcept C25642318 @default.
- W2056790168 hasConcept C2778690821 @default.
- W2056790168 hasConcept C2780385302 @default.
- W2056790168 hasConcept C41008148 @default.
- W2056790168 hasConcept C43617362 @default.
- W2056790168 hasConcept C55493867 @default.
- W2056790168 hasConcept C70721500 @default.
- W2056790168 hasConcept C71924100 @default.
- W2056790168 hasConcept C86803240 @default.
- W2056790168 hasConceptScore W2056790168C142724271 @default.
- W2056790168 hasConceptScore W2056790168C159654299 @default.
- W2056790168 hasConceptScore W2056790168C185592680 @default.
- W2056790168 hasConceptScore W2056790168C203014093 @default.
- W2056790168 hasConceptScore W2056790168C204787440 @default.
- W2056790168 hasConceptScore W2056790168C25642318 @default.
- W2056790168 hasConceptScore W2056790168C2778690821 @default.
- W2056790168 hasConceptScore W2056790168C2780385302 @default.
- W2056790168 hasConceptScore W2056790168C41008148 @default.
- W2056790168 hasConceptScore W2056790168C43617362 @default.
- W2056790168 hasConceptScore W2056790168C55493867 @default.
- W2056790168 hasConceptScore W2056790168C70721500 @default.
- W2056790168 hasConceptScore W2056790168C71924100 @default.
- W2056790168 hasConceptScore W2056790168C86803240 @default.
- W2056790168 hasIssue "1-2" @default.
- W2056790168 hasLocation W20567901681 @default.
- W2056790168 hasLocation W20567901682 @default.
- W2056790168 hasOpenAccess W2056790168 @default.
- W2056790168 hasPrimaryLocation W20567901681 @default.
- W2056790168 hasRelatedWork W2034442213 @default.
- W2056790168 hasRelatedWork W2093190478 @default.
- W2056790168 hasRelatedWork W2136134859 @default.
- W2056790168 hasRelatedWork W2157623399 @default.
- W2056790168 hasRelatedWork W2160875138 @default.
- W2056790168 hasRelatedWork W2357614251 @default.
- W2056790168 hasRelatedWork W2369633239 @default.
- W2056790168 hasRelatedWork W2392367091 @default.
- W2056790168 hasRelatedWork W140302342 @default.
- W2056790168 hasRelatedWork W2037029051 @default.
- W2056790168 hasVolume "255" @default.
- W2056790168 isParatext "false" @default.
- W2056790168 isRetracted "false" @default.
- W2056790168 magId "2056790168" @default.
- W2056790168 workType "article" @default.