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- W2056790534 abstract "Human pro-urokinase (pro-UK) was cloned into plasmid pET32b and fused to the E. coli thioredoxin (trxA). When expressed in E. coli AD494(DE3), the fusion protein Trx-pro-UK accumulated as insoluble inclusion bodies and amounted to 35% of total cellular proteins. When co-expressed with molecular chaperones human protein disulfide isomerase (PDI) and E. coli GroESL, all the expressed products still existed in the form of insoluble inclusion bodies." @default.
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- W2056790534 date "1998-10-01" @default.
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- W2056790534 title "Fusion expression of human pro-urokinase with E. coli thioredoxin" @default.
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- W2056790534 doi "https://doi.org/10.1080/15216549800204002" @default.
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