FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056848164> ?p ?o ?g. }

• W2056848164 endingPage "99" @default.
• W2056848164 startingPage "89" @default.
• W2056848164 abstract "The binding of estrogen receptor (ER) to the upstream regions of estrogen-responsive genes, the estrogen response elements (ERE), is of fundamental importance in the regulation of gene expression by estradiol. Multiple cell-specific factors affect ER-ERE binding and modulate the responses of estradiol. We studied the role of polyamines in the recognition of ER, a ligand-activated transcription factor, with a left-handed Z-DNA forming polynucleotide as well as with a plasmid containing ERE. Polyamines are cellular organic cations with multiple functions in cell growth and differentiation. Polyamines induce Z-DNA conformation in alternating purine-pyrimidine sequences. To understand the role of polyamine-induced DNA conformational transition in ER-DNA interaction, we studied the binding of partially purified rabbit uterine ER to poly(dG-m5dC).poly(dG-m5dC). The induction of Z-DNA in the polynucleotide was monitored by circular dichroism and ultraviolet spectroscopic measurements. Binding of ER to poly(dG-m5dC).poly(dG-m5dC) increased from 15% to approx. 50–60% in the presence of 7.5 mM putrescine, 0.5 mM spermidine or 0.25 mM spermine. Maximal binding of ER to the polynucleotide was observed near the midpoint of the B-DNA to Z-DNA transition of the polynucleotide. N1-acetyl spermidine and N1-acetyl spermine facilitated the B-DNA to Z-DNA transition and the binding of ER although they were less effective than the unacetylated analog. Co(NH3)63+, a trivalent inorganic cation, also provoked the B-DNA to Z-DNA transition of the polynucleotide and increased its binding to ER. At higher polyamine concentrations, there was an inhibition of ER binding to the polynucleotide. In the presence of polyamines, the binding of ER to a plasmid containing ERE was 2–3-fold higher than that to a control plasmid devoid of ERE. Polyamine-induced facilitation of ER-ERE binding was also confirmed by gel mobility shift assay. Our data indicate that conformational perturbations, similar to that of the early stages of B-DNA to Z-DNA transition, are important in the recognition of ER and ERE." @default.
• W2056848164 created "2016-06-24" @default.
• W2056848164 creator A5032174711 @default.
• W2056848164 creator A5038698115 @default.
• W2056848164 creator A5070205318 @default.
• W2056848164 date "1995-08-01" @default.
• W2056848164 modified "2023-10-10" @default.
• W2056848164 title "Polyamine-mediated conformational perturbations in DNA alter the binding of estrogen receptor to poly(dG-m5dC).poly(dG-m5dC) and a plasmid containing the estrogen response element" @default.
• W2056848164 cites W1532829068 @default.
• W2056848164 cites W1549730489 @default.
• W2056848164 cites W1595544422 @default.
• W2056848164 cites W1775749144 @default.
• W2056848164 cites W1870380925 @default.
• W2056848164 cites W1906197854 @default.
• W2056848164 cites W1963805722 @default.
• W2056848164 cites W1963849454 @default.
• W2056848164 cites W1974032247 @default.
• W2056848164 cites W1979158254 @default.
• W2056848164 cites W1981224408 @default.
• W2056848164 cites W1988969508 @default.
• W2056848164 cites W1995929927 @default.
• W2056848164 cites W1999999697 @default.
• W2056848164 cites W2005689930 @default.
• W2056848164 cites W2009056360 @default.
• W2056848164 cites W2012750519 @default.
• W2056848164 cites W2021592164 @default.
• W2056848164 cites W2028507406 @default.
• W2056848164 cites W2033108297 @default.
• W2056848164 cites W2040347789 @default.
• W2056848164 cites W2048595449 @default.
• W2056848164 cites W2049327026 @default.
• W2056848164 cites W2052333294 @default.
• W2056848164 cites W2056632912 @default.
• W2056848164 cites W2062728363 @default.
• W2056848164 cites W2063795328 @default.
• W2056848164 cites W2078265622 @default.
• W2056848164 cites W2078500258 @default.
• W2056848164 cites W2082278833 @default.
• W2056848164 cites W2085124463 @default.
• W2056848164 cites W2087246825 @default.
• W2056848164 cites W2090353848 @default.
• W2056848164 cites W2093108758 @default.
• W2056848164 cites W2132185105 @default.
• W2056848164 cites W2147427274 @default.
• W2056848164 cites W2156465612 @default.
• W2056848164 cites W2322544278 @default.
• W2056848164 cites W260259550 @default.
• W2056848164 cites W4238270754 @default.
• W2056848164 doi "https://doi.org/10.1016/0960-0760(95)00126-k" @default.
• W2056848164 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7662593" @default.
• W2056848164 hasPublicationYear "1995" @default.
• W2056848164 type Work @default.
• W2056848164 sameAs 2056848164 @default.
• W2056848164 citedByCount "47" @default.
• W2056848164 countsByYear W20568481642012 @default.
• W2056848164 countsByYear W20568481642015 @default.
• W2056848164 countsByYear W20568481642016 @default.
• W2056848164 countsByYear W20568481642018 @default.
• W2056848164 countsByYear W20568481642019 @default.
• W2056848164 countsByYear W20568481642020 @default.
• W2056848164 countsByYear W20568481642021 @default.
• W2056848164 countsByYear W20568481642023 @default.
• W2056848164 crossrefType "journal-article" @default.
• W2056848164 hasAuthorship W2056848164A5032174711 @default.
• W2056848164 hasAuthorship W2056848164A5038698115 @default.
• W2056848164 hasAuthorship W2056848164A5070205318 @default.
• W2056848164 hasBestOaLocation W20568481641 @default.
• W2056848164 hasConcept C111921641 @default.
• W2056848164 hasConcept C121608353 @default.
• W2056848164 hasConcept C133571119 @default.
• W2056848164 hasConcept C138885662 @default.
• W2056848164 hasConcept C153911025 @default.
• W2056848164 hasConcept C179926584 @default.
• W2056848164 hasConcept C181199279 @default.
• W2056848164 hasConcept C185592680 @default.
• W2056848164 hasConcept C2776109719 @default.
• W2056848164 hasConcept C2777028655 @default.
• W2056848164 hasConcept C2777497464 @default.
• W2056848164 hasConcept C2780115381 @default.
• W2056848164 hasConcept C41895202 @default.
• W2056848164 hasConcept C530470458 @default.
• W2056848164 hasConcept C54355233 @default.
• W2056848164 hasConcept C552990157 @default.
• W2056848164 hasConcept C55493867 @default.
• W2056848164 hasConcept C77122620 @default.
• W2056848164 hasConcept C84606932 @default.
• W2056848164 hasConcept C86803240 @default.
• W2056848164 hasConceptScore W2056848164C111921641 @default.
• W2056848164 hasConceptScore W2056848164C121608353 @default.
• W2056848164 hasConceptScore W2056848164C133571119 @default.
• W2056848164 hasConceptScore W2056848164C138885662 @default.
• W2056848164 hasConceptScore W2056848164C153911025 @default.
• W2056848164 hasConceptScore W2056848164C179926584 @default.
• W2056848164 hasConceptScore W2056848164C181199279 @default.
• W2056848164 hasConceptScore W2056848164C185592680 @default.
• W2056848164 hasConceptScore W2056848164C2776109719 @default.
• W2056848164 hasConceptScore W2056848164C2777028655 @default.
• W2056848164 hasConceptScore W2056848164C2777497464 @default.

• next