Matches in SemOpenAlex for { <https://semopenalex.org/work/W2056849791> ?p ?o ?g. }
- W2056849791 endingPage "F763" @default.
- W2056849791 startingPage "F751" @default.
- W2056849791 abstract "Using patch clamp, we induced depolarization of descending vasa recta (DVR) pericytes or endothelia and tested whether it was conducted to distant cells. Membrane potential was measured with the fluorescent voltage dye di-8-ANEPPS or with a second patch-clamp electrode. Depolarization of an endothelial cell induced responses in other endothelia within a millisecond and was slowed by gap junction blockade with heptanol. Endothelial response to pericyte depolarization was poor, implying high-resistance myo-endothelial coupling. In contrast, dual patch clamp of neighboring pericytes revealed syncytial coupling. At high sampling rate, the spread of depolarization between pericytes and endothelia occurred in 9 ± 2 or 12 ± 2 μs, respectively. Heptanol (2 mM) increased the overall input resistance of the pericyte layer to current flow and prevented transmission of depolarization between neighboring cells. The fluorescent tracer Lucifer yellow (LY), when introduced through ruptured patches, spread between neighboring endothelia in 1 to 7 s, depending on location of the flanking cell. LY diffused to endothelial cells on the ipsilateral but not contralateral side of the DVR wall and minimally between pericytes. We conclude that both DVR pericytes and endothelia are part of individual syncytia. The rate of conduction of membrane potential exceeds that for diffusion of hydrophilic molecules by orders of magnitude. Gap junction coupling of adjacent endothelial cells may be spatially oriented to favor longitudinal transmission along the DVR axis." @default.
- W2056849791 created "2016-06-24" @default.
- W2056849791 creator A5016230294 @default.
- W2056849791 creator A5019713148 @default.
- W2056849791 creator A5021269788 @default.
- W2056849791 creator A5064106413 @default.
- W2056849791 creator A5087634027 @default.
- W2056849791 date "2014-04-01" @default.
- W2056849791 modified "2023-10-13" @default.
- W2056849791 title "Descending vasa recta endothelial cells and pericytes form mural syncytia" @default.
- W2056849791 cites W1487735792 @default.
- W2056849791 cites W1545180485 @default.
- W2056849791 cites W1562654579 @default.
- W2056849791 cites W1571477720 @default.
- W2056849791 cites W1746005614 @default.
- W2056849791 cites W1968788883 @default.
- W2056849791 cites W1971990947 @default.
- W2056849791 cites W1983356028 @default.
- W2056849791 cites W1984746596 @default.
- W2056849791 cites W2000010931 @default.
- W2056849791 cites W2000290070 @default.
- W2056849791 cites W2005284425 @default.
- W2056849791 cites W2015151692 @default.
- W2056849791 cites W2016606880 @default.
- W2056849791 cites W2018128965 @default.
- W2056849791 cites W2018981570 @default.
- W2056849791 cites W2034836569 @default.
- W2056849791 cites W2034948022 @default.
- W2056849791 cites W2037737168 @default.
- W2056849791 cites W2041430298 @default.
- W2056849791 cites W2044458918 @default.
- W2056849791 cites W2049338620 @default.
- W2056849791 cites W2051270707 @default.
- W2056849791 cites W2053695091 @default.
- W2056849791 cites W2077128217 @default.
- W2056849791 cites W2084099919 @default.
- W2056849791 cites W2085784194 @default.
- W2056849791 cites W2088233769 @default.
- W2056849791 cites W2089504645 @default.
- W2056849791 cites W2093958843 @default.
- W2056849791 cites W2102274437 @default.
- W2056849791 cites W2107108081 @default.
- W2056849791 cites W2108828024 @default.
- W2056849791 cites W2112254943 @default.
- W2056849791 cites W2114925922 @default.
- W2056849791 cites W2117954351 @default.
- W2056849791 cites W2118454019 @default.
- W2056849791 cites W2123209321 @default.
- W2056849791 cites W2129508153 @default.
- W2056849791 cites W2137727376 @default.
- W2056849791 cites W2139176711 @default.
- W2056849791 cites W2139720121 @default.
- W2056849791 cites W2143948871 @default.
- W2056849791 cites W2147611167 @default.
- W2056849791 cites W2153040252 @default.
- W2056849791 cites W2153457849 @default.
- W2056849791 cites W2154509425 @default.
- W2056849791 cites W2154870960 @default.
- W2056849791 cites W2164181618 @default.
- W2056849791 cites W2169726777 @default.
- W2056849791 cites W2172094535 @default.
- W2056849791 cites W2281229447 @default.
- W2056849791 cites W2317113741 @default.
- W2056849791 cites W2329628078 @default.
- W2056849791 cites W2416635055 @default.
- W2056849791 cites W2465379362 @default.
- W2056849791 cites W4211091257 @default.
- W2056849791 doi "https://doi.org/10.1152/ajprenal.00470.2013" @default.
- W2056849791 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3962606" @default.
- W2056849791 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24381184" @default.
- W2056849791 hasPublicationYear "2014" @default.
- W2056849791 type Work @default.
- W2056849791 sameAs 2056849791 @default.
- W2056849791 citedByCount "7" @default.
- W2056849791 countsByYear W20568497912014 @default.
- W2056849791 countsByYear W20568497912016 @default.
- W2056849791 countsByYear W20568497912018 @default.
- W2056849791 countsByYear W20568497912019 @default.
- W2056849791 countsByYear W20568497912021 @default.
- W2056849791 crossrefType "journal-article" @default.
- W2056849791 hasAuthorship W2056849791A5016230294 @default.
- W2056849791 hasAuthorship W2056849791A5019713148 @default.
- W2056849791 hasAuthorship W2056849791A5021269788 @default.
- W2056849791 hasAuthorship W2056849791A5064106413 @default.
- W2056849791 hasAuthorship W2056849791A5087634027 @default.
- W2056849791 hasBestOaLocation W20568497912 @default.
- W2056849791 hasConcept C105702510 @default.
- W2056849791 hasConcept C123012128 @default.
- W2056849791 hasConcept C12554922 @default.
- W2056849791 hasConcept C131584629 @default.
- W2056849791 hasConcept C147944092 @default.
- W2056849791 hasConcept C158157758 @default.
- W2056849791 hasConcept C169760540 @default.
- W2056849791 hasConcept C177779419 @default.
- W2056849791 hasConcept C181911157 @default.
- W2056849791 hasConcept C185263204 @default.
- W2056849791 hasConcept C185592680 @default.
- W2056849791 hasConcept C191897082 @default.