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• W2056857250 abstract "Barcode-tagged PCR primers used for multiplex amplicon sequencing generate a thus-far-overlooked amplification bias that produces variable terminal restriction fragment length polymorphism (T-RFLP) and pyrosequencing data from the same environmental DNA template. We propose a simple two-step PCR approach that increases reproducibility and consistently recovers higher genetic diversity in pyrosequencing libraries." @default.
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• W2056857250 date "2012-01-15" @default.
• W2056857250 modified "2023-10-16" @default.
• W2056857250 title "Barcoded Primers Used in Multiplex Amplicon Pyrosequencing Bias Amplification" @default.
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• W2056857250 doi "https://doi.org/10.1128/aem.07448-11" @default.
• W2056857250 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3255734" @default.
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