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- W2056918373 abstract "A fused-silica capillary that is wall-modified, via chemically bonding histidine functional groups to the capillary wall, is described. With benzyl alcohol as neutral marker, the electroosmotic flow (EOF) of the bonded phase is reversed and the migration velocity increases slightly as the pH decreases. Changes in the type, concentration, and pH of the buffer, as well as the applied voltage, were performed to determine the optimum condition for the simultaneous separation of neutral and anionic plant phenols. A separation of (+)-catechin, gallic acid, caffeic acid, (−)-epicatechin, salicylic acid, quercetin, and p-coumaric acid could be obtained on a 100 cm × 75 μm I.D. column under acetate buffer (10 mM, pH 5.0) and an applied voltage of −20 kV with good peak shapes and efficiencies over the range 7.46 × 105 to 3.86 × 106 plates per meter. The amount of plant phenols in wines has also been determined with the established method. Quantitative analysis was performed by the standard addition method. The mechanism of the separation of the analytes is based on a combination of differences in electrophoretic mobility and solute/attached moiety interactions." @default.
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- W2056918373 date "2002-11-03" @default.
- W2056918373 modified "2023-10-13" @default.
- W2056918373 title "APPLICATION OF HISTIDINE-BONDED PHASE CAPILLARY COLUMN TO ELECTROPHORETIC SEPARATION OF PLANT PHENOLS" @default.
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- W2056918373 doi "https://doi.org/10.1081/ci-100108770" @default.
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