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• W2056945166 abstract "<b><i>Objective:</i></b> We report the infection of New Zealand white rabbits with Epstein-Barr virus (EBV). <b><i>Methods:</i></b> EBV prepared in B95-8 (producer) cells was inoculated to rabbits by combined intranasal and oral routes. Blood and white blood cell (WBC) samples were taken before infection, then on days 8, 28 and 98 post-infection (p.i.). <b><i>Results:</i></b> Administration of either 3 × 10⁸ (group A, 11 rabbits) or 1 × 10⁹ (group B, 10 rabbits) EBV DNA copies per animal induced subacute and/or persistent infection. The IgG antibodies in plasma were detected by ELISA as well as by immunoblot (IB). The IB bands showed mainly antibodies to the BZRF1/Zta transactivation polypeptide (69.2%), the p54 early protein (53.4%) and to the p23 capsid protein (35.8%). No anti-EBNA1 antibody was detected throughout. Viral DNA could be detected by PCR in WBCs and/or spleen of 7 out of 21 infected rabbits (30%), while 60-80% of them showed serologic response. The transiently present EBV DNA was accompanied by LMP1 antigen. <b><i>Conclusions:</i></b> Rabbits developed persistent EBV infection in the absence of EBNA1 antibodies and by the lack of typical infectious mononucleosis-like syndrome. The absence of EBNA1 antibody may reflect the lack of EBNA1 in B cells of EBV-inoculated rabbits." @default.
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• W2056945166 date "2014-01-01" @default.
• W2056945166 modified "2023-09-26" @default.
• W2056945166 title "Epstein-Barr Virus (HHV-4) Inoculation to Rabbits by Intranasal and Oral Routes Results in Subacute and/or Persistent Infection Dissimilar to Human Disease" @default.
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• W2056945166 doi "https://doi.org/10.1159/000360223" @default.
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