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- W2056979172 abstract "We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immuno-PCR is reproducible, with a larger dynamic range and a sensitivity several orders of magnitude greater than the corresponding conventional ELISA. The minimum concentration of analyte detected was of the order of 1 pg/mL These characteristics should contribute to the adoption of immuno-PCR by research and clinical laboratories." @default.
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- W2056979172 date "2009-06-30" @default.
- W2056979172 modified "2023-09-24" @default.
- W2056979172 title "A Novel Immuno-Polymerase Chain Reaction Protocol Incorporating a Highly Purified Streptavidin-DNA Conjugate" @default.
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- W2056979172 doi "https://doi.org/10.1080/15321810903084764" @default.
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