FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2057083513> ?p ?o ?g. }

• W2057083513 endingPage "1656" @default.
• W2057083513 startingPage "1651" @default.
• W2057083513 abstract "Hybrid complexes of proteins and colloidal semiconductor nanocrystals (quantum dots, QDs) are of increasing interest in various fields of biochemistry and biomedicine, for instance for biolabeling or drug transport. The usefulness of protein-QD complexes for such applications is dependent on the binding specificity and strength of the components. Often the binding properties of these components are difficult and time consuming to assess.In this work we characterized the interaction between recombinant light harvesting chlorophyll a/b complex (LHCII) and CdTe/CdSe/ZnS QDs by using ultracentrifugation and fluorescence resonance energy transfer (FRET) assay experiments. Ultracentrifugation was employed as a fast method to compare the binding strength between different protein tags and the QDs. Furthermore the LHCII:QD stoichiometry was determined by separating the protein-QD hybrid complexes from unbound LHCII via ultracentrifugation through a sucrose cushion.One trimeric LHCII was found to be bound per QD. Binding constants were evaluated by FRET assays of protein derivatives carrying different affinity tags. A new tetra-cysteine motif interacted more strongly (Ka=4.9±1.9nM(-1)) with the nanoparticles as compared to a hexahistidine tag (His6 tag) (Ka~1nM(-1)).Relative binding affinities and binding stoichiometries of hybrid complexes from LHCII and quantum dots were identified via fast ultracentrifugation, and binding constants were determined via FRET assays.The combination of rapid centrifugation and fluorescence-based titration will be useful to assess the binding strength between different types of nanoparticles and a broad range of proteins." @default.
• W2057083513 created "2016-06-24" @default.
• W2057083513 creator A5052696714 @default.
• W2057083513 creator A5053234783 @default.
• W2057083513 creator A5058825629 @default.
• W2057083513 creator A5062747828 @default.
• W2057083513 creator A5090721101 @default.
• W2057083513 date "2014-06-01" @default.
• W2057083513 modified "2023-09-25" @default.
• W2057083513 title "Comparison of quantum dot-binding protein tags: Affinity determination by ultracentrifugation and FRET" @default.
• W2057083513 cites W1517168457 @default.
• W2057083513 cites W177800984 @default.
• W2057083513 cites W1955092635 @default.
• W2057083513 cites W1967321494 @default.
• W2057083513 cites W1973590723 @default.
• W2057083513 cites W1973996951 @default.
• W2057083513 cites W1991646828 @default.
• W2057083513 cites W1995400309 @default.
• W2057083513 cites W1996225771 @default.
• W2057083513 cites W1996259855 @default.
• W2057083513 cites W1998192274 @default.
• W2057083513 cites W2003589691 @default.
• W2057083513 cites W2007860356 @default.
• W2057083513 cites W2008685387 @default.
• W2057083513 cites W2014911581 @default.
• W2057083513 cites W2015355762 @default.
• W2057083513 cites W2016564174 @default.
• W2057083513 cites W2017321333 @default.
• W2057083513 cites W2022172775 @default.
• W2057083513 cites W2024113054 @default.
• W2057083513 cites W2031763560 @default.
• W2057083513 cites W2035284243 @default.
• W2057083513 cites W2037525645 @default.
• W2057083513 cites W2041630712 @default.
• W2057083513 cites W2043628224 @default.
• W2057083513 cites W2062082739 @default.
• W2057083513 cites W2064584226 @default.
• W2057083513 cites W2066262062 @default.
• W2057083513 cites W2068119209 @default.
• W2057083513 cites W2076331684 @default.
• W2057083513 cites W2079433440 @default.
• W2057083513 cites W2080262010 @default.
• W2057083513 cites W2094425913 @default.
• W2057083513 cites W2120765541 @default.
• W2057083513 cites W2138032051 @default.
• W2057083513 cites W2151994158 @default.
• W2057083513 cites W2154536694 @default.
• W2057083513 cites W2162444849 @default.
• W2057083513 cites W2317976500 @default.
• W2057083513 cites W2330125178 @default.
• W2057083513 cites W41325891 @default.
• W2057083513 doi "https://doi.org/10.1016/j.bbagen.2013.11.025" @default.
• W2057083513 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24361618" @default.
• W2057083513 hasPublicationYear "2014" @default.
• W2057083513 type Work @default.
• W2057083513 sameAs 2057083513 @default.
• W2057083513 citedByCount "7" @default.
• W2057083513 countsByYear W20570835132015 @default.
• W2057083513 countsByYear W20570835132017 @default.
• W2057083513 countsByYear W20570835132018 @default.
• W2057083513 countsByYear W20570835132019 @default.
• W2057083513 countsByYear W20570835132020 @default.
• W2057083513 crossrefType "journal-article" @default.
• W2057083513 hasAuthorship W2057083513A5052696714 @default.
• W2057083513 hasAuthorship W2057083513A5053234783 @default.
• W2057083513 hasAuthorship W2057083513A5058825629 @default.
• W2057083513 hasAuthorship W2057083513A5062747828 @default.
• W2057083513 hasAuthorship W2057083513A5090721101 @default.
• W2057083513 hasConcept C121332964 @default.
• W2057083513 hasConcept C124657808 @default.
• W2057083513 hasConcept C12554922 @default.
• W2057083513 hasConcept C171250308 @default.
• W2057083513 hasConcept C185592680 @default.
• W2057083513 hasConcept C192562407 @default.
• W2057083513 hasConcept C2910425616 @default.
• W2057083513 hasConcept C54689828 @default.
• W2057083513 hasConcept C55493867 @default.
• W2057083513 hasConcept C62520636 @default.
• W2057083513 hasConcept C86803240 @default.
• W2057083513 hasConcept C91881484 @default.
• W2057083513 hasConcept C96305047 @default.
• W2057083513 hasConceptScore W2057083513C121332964 @default.
• W2057083513 hasConceptScore W2057083513C124657808 @default.
• W2057083513 hasConceptScore W2057083513C12554922 @default.
• W2057083513 hasConceptScore W2057083513C171250308 @default.
• W2057083513 hasConceptScore W2057083513C185592680 @default.
• W2057083513 hasConceptScore W2057083513C192562407 @default.
• W2057083513 hasConceptScore W2057083513C2910425616 @default.
• W2057083513 hasConceptScore W2057083513C54689828 @default.
• W2057083513 hasConceptScore W2057083513C55493867 @default.
• W2057083513 hasConceptScore W2057083513C62520636 @default.
• W2057083513 hasConceptScore W2057083513C86803240 @default.
• W2057083513 hasConceptScore W2057083513C91881484 @default.
• W2057083513 hasConceptScore W2057083513C96305047 @default.
• W2057083513 hasIssue "6" @default.
• W2057083513 hasLocation W20570835131 @default.
• W2057083513 hasLocation W20570835132 @default.
• W2057083513 hasOpenAccess W2057083513 @default.

• next